Gershenfeld1: Drug study: Imipramine effects in 12 inbred strains of mice measured by tail suspension, light-dark transition, and open field tests (2003)

Liu X, Gershenfeld HK. Genetic differences in the tail-suspension test and its relationship to imipramine response among 11 inbred strains of mice. Biol Psychiatry. 2001 Apr 1;49(7):575-81.   PubMed 11297714  

Liu X, Gershenfeld HK. An exploratory factor analysis of the Tail Suspension Test in 12 inbred strains of mice and an F2 intercross. Brain Res Bull. 2003 May 15; 60(3): 223-31.   PubMed 12754084  


         
Notes
A supplemental data set (5 strains) is also available. It is connected with the publication Liu X, Peprah D, Gershenfeld HK   Tail-suspension induced hyperthermia: a new measure of stress reactivity.

Gershenfeld1 downloads
• Download Gershenfeld1 project data set     one row per animal, one column per trait
• Download Gershenfeld1 strain means, SD, N, etc.     one row per strain/sex/measure
• Gershenfeld1 supplementary data
Participants Howard K Gershenfeld       University of Texas - Southwestern Dallas, TX
With: Liu X
ContactHoward K Gershenfeld     Howard.Gershenfeld@UTSouthwestern.edu
AcknowledgementsFunding provided by NIH MH58882; National Association for Research on Schizophrenia and Depression Young Investigators Award; Southwestern Medical Foundation; KZA Hope Fund
Project type Phenotype strain survey data set
MPD identifiersGershenfeld1     MPD:162
Data changelog No updates/corrections.       Initial release date: 07/2007.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
An investigation of stress response and sensitivity to antidepressant/anxiolytic effect of imipramine. Animals were evaluated for "stress reactivity" using the tail suspension test. They were measured for "exploratory fear" behavior using the light-dark transition and open field tests. Imipramine was administered to alleviate stress arising from immobility. There were two cohorts tested at baseline and again following i.p. injection: 1) controls with saline i.p.; 2) treated with imipramine 30 mg/kg body weight i.p.

Summary of procedures that were conducted
• tail suspension test Immobility. Baseline (saline) vs. imipramine i.p. 6 min test.
• light-dark box Anxiety-related behavior. Baseline (saline) vs. imipramine i.p. 10 min test.
• open field test Locomotor activity, exploratory and anxiety-related behavior. Baseline (saline) vs. imipramine i.p.
Mice: inbred   12 strains   ♂   age 5-10wks   2 cohorts

Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Gershenfeld1 16201 tail suspension test (none) TST duration of immobility, effect of saline injection on control group   [s]     before injection    after injection inbred 12 m 5-10wks N=5
    Gershenfeld1 16203 tail suspension test imipramine TST duration of immobility, treatment group   [s]     baseline    imipramine 30mg/kg inbred 12 m 5-10wks N=5
    Gershenfeld1 16205 tail suspension test imipramine TST immobility percentage, imipramine vs. saline   [%]  TST_immobility_pct inbred 12 m 5-10wks N=5
    Gershenfeld1 16210 light-dark box imipramine number of transitions between light and dark compartments in 10 min   [n]     baseline    imipramine 30mg/kg inbred 12 m 5-10wks N=5
    Gershenfeld1 16212 open field test imipramine distance traveled in 5 min   [cm]     baseline    imipramine 30mg/kg inbred 12 m 5-10wks N=5
    Gershenfeld1 16214 open field test imipramine vertical movements (rearing) in 15 min   [n]     baseline    imipramine 30mg/kg inbred 12 m 5-10wks N=5
    Gershenfeld1 16216 open field test imipramine average center time, 2 trials   [s]     baseline    imipramine 30mg/kg inbred 12 m 5-10wks N=5
    Gershenfeld1 16218 open field test imipramine number of fecal boli in 15 min   [n]     baseline    imipramine 30mg/kg inbred 12 m 5-10wks N=5
    All boxes