Procedure: Dual Energy X-Ray Absorptiometry (DXA) and X-Ray
Mice are anesthetized with ketamine-xylazine (i.p.) and monitored until they become unconscious.
Mice are weighed and the length of the mouse is measured with a disinfected ruler (measure length starting from the nose at 0 cm to the beginning of the tail (accuracy within 0.1 cm)).
The mouse is placed in the DXA analyzer (calibrated daily using phantoms from the manufacturer) and a snout scan is performed.
The area of interest is optimized and a measure-scan performed (exposure dose is 300 µSv).
Regions of interest are defined for data analysis (whole body excluding the head).
The mouse may go directly to the X-Ray machine (below). If not, the mouse is removed (once the image has been captured) and is placed on a heated mat (37°C) in a clean cage and monitored closely until consciousness is regained.
Raw data are transformed by accompanying software into bone and non-bone components, and fat and lean mass are determined.
An anesthetized (ketamine-xylazine) mouse is mounted onto an X-Ray permeable plate; the tail is curved along the left side of the body (used to enable identification of the X-Ray as dorsal or ventral).
The plate is inserted into the X-Ray machine.
The machine is set to a voltage of 25 kV with an integration time of 40 ms.
An image is taken using the iX-Pect software.
The mouse is removed and placed on a heated mat (37°C), in a clean cage and monitored closely until consciousness is fully gained.
Total tissue mass
Fat tissue mass
Lean tissue mass
Bone mineral density
Number of lumbar vertebrae
* Many other X-Ray parameters are available in the Supplemental data file (see GMC18 Downloads)