GMC16 project protocol
Clinical blood chemistry for 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Rathkolb B, Wolf E, Lengger C, Kollmus H, Schughart K
German Mouse Clinic: Phenotyping Pipeline
Clinical blood chemistry: 16-17 and 20-21 wks of age
||Body weight measured
||Samples processed and analyzed
||Clinical chemistry analyzer
||Clinical chemistry parameters
- Balance: Ohaus (AV213C)
- Lithium-heparin blood collection tubes
- Clinical chemistry analyzer: Beckman-Coulter (AU480)
- Beckman-Coulter System reagents
Procedure: Clinical chemistry
- Mice (non-fasting) are anesthetized via isoflurane inhalation.
- Blood is collected in the morning by retro-orbital puncture (~200 µL) in lithium heparin-coated tubes, and stored at room temperature for 1-2h maximum.
- Samples are centrifuged for 10 min at 5000 x g in a refrigerated centrifuge set at 8°C.
- Analysis is optimally done the day of blood collection (when not possible, samples are frozen at -20°C until analysis); all samples are run at room temperature.
- If necessary, samples are diluted 1:2 in deionized water.
- Samples must be free of fibrin clots (can remove with wooden applicator stick).
- Samples are run according to manufacturer's instructions.
- Plasma unsaturated iron binding capacity
See GMC16 Downloads.