Heart rate parameters, duration of intervals, amplitude of signals
Electrocardiogram: Mouse Specifics, Inc. (ECGenie), Framingham MA
Software: LabChart ECGenie/eMouse
Procedure: Electrocardiogram (ECG)
Lead plates are snapped into place onto the top of the pre-amplifier tower; the covering is removed to reveal three gel coated pads surrounded by a sticking plate; the plate is covered with the extra cover in the package.
The combined amplifier and the pre-amplifier tower are turned on.
The icon ECG acquisition on the acquisition computer is doubled clicked.
The ECG set up file is opened.
A mouse is placed on the pad, lowering the Red Acrylic Cubby to surround the mouse on 3 sides, discouraging escape.
Acquisition begins by pressing 'Start'.
The reading is stopped after 5-10 min of acquisition; there will be one long reading.
Save the data.
Follow manufacturer's instructions for using the software for data analysis.
Heart rate variability
Heart rate coefficient of variance
Duration of RR interval
Duration of PQ interval
Duration of PR interval
Duration of QRS interval
Duration of QT interval
Duration of ST interval
Duration of QTc interval
QT rate-corrected dispersion
Root mean square of successive differences of neighboring RR intervals (rMSSD)
Proportion of number of pairs of successive beat-to-beat intervals that exceed 50 ms (pNN50)