GMC13 animal documentation

Heart function assessed by electrocardiography in 6 inbred founder strains of the Collaborative Cross   (2020)

German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Moreth K, Spielmann N, Bekeredjian R, Kattus H, Lengger C, Kollmus H, Schughart K

GMC13 protocol

Animal documentation at breeding facility (HZI)

Source	Breeders obtained from The Jackson Laboratory. Mice were subsequently bred at the Helmholtz Centre for Infection Research, Braunschweig, Germany.
Ages	Mice born and weaned at HZI; shipped to test facility (below) at 7 wks of age
Feed	Ssniff (V 1534-300), provided ad libitum
Water	Acidified water (pH 3.0), provided ad libitum
Temperature	22 +/-1°C
Humidity	55% +/- 5%
Housing	Aspen wood bedding in IVC cages (Techniplast, Blue Line); 3-4 animals per cage in rooms with 192 cages
Photoperiod	12h:12h light-dark cycle
Health status	Mice maintained under specific pathogen free conditions
Care and Use	Mice maintained according to German animal welfare law

 

Animal documentation at test facility (GMC)

Source	Mice were bred at the Helmholtz Centre for Infection Research, Braunschweig, Germany (above)
Ages	Mice were shipped at 7 wks of age to test facility (Helmholtz Zentrum München, National Research Center for Environmental Health, German Mouse Clinic, Neuherberg, Germany)
Feed	Altromin 1314 (Altromin, Lage, Germany), provided ad libitum
Water	UV sterilized and sterile filtered water, provided ad libitum
Temperature	22 +/-2°C
Humidity	55%
Housing	Techniplast Greenline GM500 cages (IVC); up to 5 mice per cage
Photoperiod	12h:12h light-dark cycle
Care and Use	All procedures approved by the government of Upper Bavaria, Germany (144-10)