The test is performed at room temperature (23 °C) with a 12:12h light/dark cycle (lights on 0600, lights off 1800h). Paper tissue is provided as bedding material.
Each mouse is placed individually in the chamber for a period of 21 h (from 1300 to 1000h next day) with free access to food and water. Metabolic cuvettes are set up in a ventilated cabinet continuously supplied with an overflow of fresh air from outside.
Body weight before and after testing
Oxygen consumption (VO2)
Carbon dioxide production (VCO2)
Respiratory exchange rate (RER = VCO2/VO2)
Heat production (HP = (4.44 + 1.43 x RER) x VO2))
Data were collected in 15-min bins over the 21-hour period, giving a total of 84 readings for each parameter. All data are available in the Supplemental data file (see Downloads on the GMC09 project page). MPD computed total food and water intake (for the 21-h test period) and computed means for the additional parameters across all 15-min bins.