GMC09 animal documentation

Energy metabolism evaluated through indirect calorimetry in 8 inbred founder strains of the Collaborative Cross   (2020)

German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Rozman J, Klingenspor M, Lengger C, Kollmus H, Schughart K




Animal documentation at breeding facility (HZI)

Source Breeders obtained from The Jackson Laboratory. Mice were subsequently bred at the Helmholtz Centre for Infection Research, Braunschweig, Germany.
Ages
Mice born and weaned at HZI; shipped to test facility (below) at 7 wks of age
Feed

Ssniff (V 1534-300), provided ad libitum
Water
Acidified water (pH 3.0), provided ad libitum
Temperature
22 +/-1°C
Humidity
55% +/- 5%
Housing
Aspen wood bedding in IVC cages (Techniplast, Blue Line); 3-4 animals per cage in rooms with 192 cages
Photoperiod 12h:12h light-dark cycle
Health status Mice maintained under specific pathogen free conditions
Care and Use

Mice maintained according to German animal welfare law

 

Animal documentation at test facility (GMC)

Source Mice were bred at the Helmholtz Centre for Infection Research, Braunschweig, Germany (above)
Ages
Mice were shipped at 7 wks of age to test facility (Helmholtz Zentrum München, National Research Center for Environmental Health, German Mouse Clinic, Neuherberg, Germany)
Feed

Altromin 1314 (Altromin, Lage, Germany), provided ad libitum
Water
UV sterilized and sterile filtered water, provided ad libitum
Temperature
22 +/-2°C
Humidity
55%
Housing
Techniplast Greenline GM500 cages (IVC); up to 5 mice per cage
Photoperiod 12h:12h light-dark cycle
Care and Use

All procedures approved by the government of Upper Bavaria, Germany (144-10)