GMC08 project protocol
Transepidermal water loss in 8 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Aguilar-Pimentel A, Ollert M, Lengger C, Kollmus H, Schughart K
GMC08 animal documentation
German Mouse Clinic: Phenotyping Pipeline
Transepidermal water loss test: 11 - 12 wks of age
Project
protocol
-
Contents
Workflow
and
sampling
Step
Procedure
Equipment
Data
collected
1
Transepidermal water loss test
Condensor chamber
Transepidermal water loss, coefficient of variation, skin surface water loss
Equipment
and
supplies
Condensor chamber: Tewameter (AquaFlux AF200)
Procedure: Transepidermal water loss (TEWL) test
Each mouse is analyzed in a non-invasive manner with a special Tewameter which is placed on the skin (non-shaved).
Within 60-90 s of surface contact, measurements are available and recorded.
The TEWL data were normalized by calculating the mean TEWL performed per day which was substracted from the measured TEWL value.
Data
collected
by
investigator
Transepidermal water loss (TEWL), normalized
Coefficient of variation of TEWL
Skin surface water loss