GMC04 project protocol
Motor coordination and balance assessed by the rotarod test in 7 inbred founder strains of the Collaborative Cross (2020)
German Mouse Clinic and Department of Infection Genetics, HZI
With: Hrabě de Angelis M, Fuchs H, Gailus-Durner V, Becker L, Klopstock T, Lengger C, Kollmus H, Schughart K
See also: GMC04 animal documentation
German Mouse Clinic: Phenotyping Pipeline
Rotarod: 9-10 wks of age
Latency to fall (3 trials)
- Accelerating rotarod: Bioseb (Rota-Rod/RS LE8200), FRANCE
- Rod: 5 cm diameter, made of hard plastic material, covered by grey rubber foam
- Lane width: 5 cm
- Stop watch
- Balance: Ohaus (AV213C)
- Cleaning solution: 50% ethanol
- Mice are acclimated to the testing room for at least 15 min.
- Three to five mice are measured concurrently on the rod with a divider between the respective lanes.
- Each mouse is subjected to three trials separated by 15 min inter-trial intervals (ITI).
- The apparatus is set to accelerating mode from 4 to 40 rpm in 5 min.
- Mice are positioned in their respective lanes, with mice walking forward on the rod at 4 rpm.
- The start button is pressed so that the rotarod increases in speed over time.
- The latency at which each mouse falls off the rod is recorded.
- If a mouse clinging on the rod completes a full passive rotation, the timer is stopped for that mouse by pushing down the lever and latency recorded as such. In this case, passive rotation is considered a failure in performance, like falling. Make note of passive falling.
- Latency to fall from accelerating rotarod (3 trials, mean of all trials)
- Body weight