Donahue14 animal documentation

Reproductive performance of 42 BXD (RwwJ) recombinant inbred strains of mice   (2011)

Acclimation and testing periods

Supplier	BXD recombinant inbred mice were obtained from the Repository of The Jackson Laboratory (JAX®, Bar Harbor, Maine)
Ages	Mice were pair or trio-mated with siblings between 3 to 15 wks of age, and retired as breeders between 13 to 60 wks of age
Feed	Mice were fed pelleted and autoclaved 5K52 diet, modified from the NIH 31M open formula with 6% fat (LabDiet® 5K52 6% Fat, St. Louis, MO)
Water	Mice were provided ad libitum access to autoclaved acidified water (pH 2.8 to 3.1)
Housing	Mice were housed in individually ventilated micro-isolator cages from Thoren Caging Systems Inc. (Hazelton, PA) within an SPF maximum barrier room (AX 11). The room was supplied with HEPA-filtered air at 19 changes/h.
Bedding	Autoclaved pine shavings (Crobb Box, Ellsworth, ME)
Humidity	Relative humidity of 35 ± 4%
Photoperiod	12-h light: 12-h dark cycle
Temperature	22 ± 2°C
Regulatory compliance	All procedures were conducted in strict compliance with the policies on animal welfare of the National Institutes of Health and were approved by the Animal Care and Use Committee of The Jackson Laboratory