Donahue1 animal documentation

Bone mineral density, body composition, and craniofacial characterization in 30 inbred strains of mice   (2002)

Acclimation and testing periods

Ages	Mice were housed in JAX® production rooms until 4 wks of age
Feed	NIH31 diet containing 6% fat, 17% protein, plus vitamin and mineral fortification (Purina Mills International, Brentwood, MO). The full composition of this open formula diet is available at here
Water	Animal were provided free access to acidified water (pH 2.5 with HCl to retard bacterial growth)
Housing	The mice were received into our research colony at 4 weeks of age, and housed in groups of 3-4 of the same sex
Bedding	Sterilized White Pine shavings
Health status	Sentinel mice were periodically examined throughout the study and were free of microbiological infections

Note: These same mice were tested for acoustic startle response and prepulse inhibition at 5-7 weeks of age by the Willott1 project and subjected to a morphological examination of the eye at 6-7 weeks of age by the Hawes1 project.

SEA/GnJ mice of two Bmp5 genotypes were tested in this project and significant differences were found for some parameters. See comparison plots of for all measurements.