Acclimation & testing periods
Vendor Mice were obtained from The Jackson Laboratory (JAX®), Bar Harbor ME, USA Acclimation Mice were allowed to acclimate at the test facility for at least 1 wk Ages Male mice, 8–10 wks old at the start of testing Feed & water Water and Purina ProLab IsoPro 3000 were available ad libitum. For the first few days after drug pellets were implanted, food and water were positioned more accessibly near the bedding surface. Housing Mice were housed in standard 20x30 cm2 ventilated polycarbonate cages with laboratory grade Bed-O-Cob bedding. All mice from each strain were group housed 5 mice per cage, with the exception of BALB/cByJ, CAST/EiJ and SJL/J due to fighting. A small section of PVC pipe was present in each cage for enrichment. Humidity 40–50% relative humidity Photoperiod 12-h light:12-h dark schedule with lights on at 0700 h Temperature 20° - 24°C Regulatory compliance All testing procedures were conducted in strict compliance with the ‘Guide for the Care and Use of Laboratory Animals’ (Institute of Laboratory Animal Resources, National Research Council, 1996) and approved by the Institutional Animal Care and Use Committee of the University of North Carolina.