Crabbe2: Drug study: Effects of diazepam on activity, motor coordination, and body temperature, in 15 inbred strains of mice (1998)

Crabbe JC, Gallaher EJ, Cross SJ, Belknap JK. Genetic determinants of sensitivity to diazepam in inbred mice. Behav Neurosci. 1998 Jun;112(3):668-77.   PubMed 9676982  


       
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Participants John C Crabbe       Oregon Health & Science University Portland, OR
Edward J Gallaher       Oregon Health & Science University Portland, OR
John K Belknap       Oregon Health & Science University Portland, OR
With: Cross SJ
ContactJohn C Crabbe     crabbe@ohsu.edu
AcknowledgementsFunding provided by NIH DA05228, AA08621, AA10760; Department of Veterans Affairs

Acknowledgements: Jones G, Young E, Phillips E
Project type Phenotype strain survey data set
MPD identifiersCrabbe2     MPD:155
Data changelog No updates/corrections.       Initial release date: 02/2009.
Formatted citation
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A investigation of the pharmacogenetic components and behavioral sensitivity to diazepam. Animals were evaluated at baseline and following diazepam administration (doses of 2, 4, 8, 16, and 20 mg/kg body weight i.p.) in the following areas: multi-system analysis of activity and motor function, coordination and balance, drug metabolism, and thermal regulation.

Summary of procedures that were conducted
• rotarod Latency to fall from 5 rpm rotarod immediately after diazepam injection.
• drug and metabolite quantification Diazepam (and metabolite) concentration in brain tissue immediately after diazepam injection and rotarod.
• monitoring system Locomotor activity. Baseline vs. immediately after Diazepam injection (several doses). 15 min test.
• body temperature Baseline vs. 30 min after diazepam injection (several doses).
Mice: inbred   15 strains   ♀♂   age 6-7wks   5 cohorts

Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Crabbe2 15501 drug and metabolite quantification diazepam brain diazepam and metabolite concentration (30 min post-injection)   [ng/g]     8 mg/kg    16 mg/kg inbred 15 m 6-7wks N=8
    Crabbe2 15503 drug and metabolite quantification diazepam brain diazepam and metabolite concentration (<5 min post-injection) after rotarod   [ng/g]     trial block1    trial block2 inbred 8 m 6-7wks N=13
    Crabbe2 15504 monitoring system diazepam horizontal distance traveled (immediately to 15 min post-injection)   [cm]     baseline    doses mg/kg:    2    4    8    16 inbred 15 m 6-7wks N=8
    Crabbe2 15509 monitoring system diazepam vertical (rearing) activity (immediately to 15 min post-injection)   [n]     baseline    doses mg/kg:    2    4    8    16 inbred 15 m 6-7wks N=8
    Crabbe2 15514 body temperature diazepam body temperature (30 min post-injection)   [°C]     baseline    doses mg/kg:    2    4    8    16 inbred 15 m 6-7wks N=8
    Crabbe2 15519 rotarod diazepam latency to fall from 5 rpm rotarod, 0 min post-injection   [s]     trial block1    trial block2 inbred 8 m 6-7wks N=13
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