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Vendor:
Mice obtained from The Jackson Laboratory.
Health status report: The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. See JAX®Mice Quality Control Programs or the JAX®Mice Catalog for the list of agents monitored. Sentinel mice from production facility and research facility (non-SPF) rooms represented in this study were negative for all agents tested except those noted in detailed health status reports for each animal room. Shipment notes and dates: Shipping and testing were accomplished in four batches.
Shipment age: Most mice were shipped at 7 wks of age with
behavioral tests beginning at 8-10 wks of age.
Environment before shippingFeed: Purina 5K52 (6% fat diet).Water: Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/mL) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA). Housing: Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system. Bedding: Steam autoclaved white pine shavings. Photoperiod: 14 h light (generally 0430 h) 10 h dark. Temperature: 18 - 24 °C.
Relative humidity: ~50%.
Acclimation and Testing PeriodsDuration: 1-3 wks in conventional, non-barrier facility at Oregon Health and Science University, Portland Oregon, USA.Feed: Purina 5001 bought locally; ad libitum. Water: Tap; ad libitum. Housing: Usually 3 mice per cage (Thoren 1A (11" x 7" x 6") with filter tops for all strains). Bedding: 1/4" Bed-o-cob. Photoperiod: 12 h on (0600 h) 12 h off. Temperature: 21 +/- 1.0 °C. Health status report: Mice were reported in excellent health. |