Chesler5 animal documentation

Intestinal microbiota in 10 inbred strains of mice   (2012)

Chesler EJ, Bubier JA, Podar M
With: Campbell JH, Foster CM, Vishnivetskaya T, Campbell AG, Yang ZK, Wymore A, Palumbo AV

See also: Chesler5 protocol


Chesler5 Animal Documentation

Acclimation & testing periods

Vendor

The Jackson Laboratory (JAX®, Bar Harbor, ME); maintained at the Oak Ridge National Laboratory vivarium for no longer than 10 generations. Mice were bred in the ORNL facility and weaned at 3-4 wks of age and distributed with same-gender siblings

Ages
Mice tested at 8-10 wks of age
Feed
Harlan Laboratories Teklad Rodent Diet 8604 (Indianapolis IN) or Purina 5053, given ad libitum
Water
Provided ad libitum
Temperature
Not specified
Humidity
Not specified
Housing
Animal Care Systems caging (cages were under negative pressure created by the facility exhaust system (20-30 change per h; cages were exhausted through a HEPA-like filter in front of the cage and had a solid lid designed such that unfiltered room air could not enter the cage; cages opened under a Nu Aire or Baker Laminar Flow work station)
Photoperiod Not specified
Care and Use

Procedures approved by the ORNL and University of Tennessee Animal Care and Use Committee