Acclimation & testing periods
Vendor The Jackson Laboratory (JAX®, Bar Harbor, ME) Ages Mice obtained at 6 wks of age; tested 12-16 wks of age Feed NIH31 5K52, LabDiet (St. Louis MO), given ad libitum Water Acidified, provided ad libitum Temperature Climate controlled, temperature not specified Humidity Climate controlled, humidity not specified Housing Duplex polycarbonate cages with a Shepherd Shack on ventilated racks providing 99.997% HEPA filtered air; initially mice housed 5 per cage; reduced density for some males due to fighting Bedding Pine cob Photoperiod 12h:12h light cycle Care and Use Protocols approved by The Jackson Laboratory Animal Care and Use Committee, and conducted in compliance with the Niational Institutes of Health Guidelines for the Care and Use of Laboratory Animals