MPD: Chesler2: animal documentation

Chesler2 animal documentation

Multi-system assessment of 8 inbred founder strains and 54 F1 hybrids of the Collaborative Cross (2011)

Chesler EJ, Philip VM, Voy BH
With: Sokoloff G, Ackert-Bicknell C, Striz M, Churchill GA, Lariviere WR, Palmer AA, OHara BF

Chesler2_Animal

Acclimation and testing periods

Supplier
Inbred mice were obtained from The Jackson Laboratory (JAXÂ®, Bar Harbor, Maine). Collaborative Cross (CC) mice bred and obtained from Oak Ridge National Laboratory (Oak Ridge, TN 37831).

Ages variable age

Feed

Irradiated Purina 5053: 5% fat, 20% protein given ad libitum

Water

Chlorinated 3â5 p.p.m. given ad libitum; Automatic: Edstrom

Housing
Optimice Single (75 sq. in.), Thoren Single (75 sq. in.), Thoren Duplex (52 sq. in.). All polycarbonate; Thoren filter top; Nestlets, igloos, PVC pipes environmental enrichment

Bedding

Harlan Softcob bedding

Photoperiod

14:10 h L:D; light intensities maintained at 3 lux at a distance of 30 in from the floor surface

Humidity 30-70%

Temperature

21 +/- 1.0°C

Health status
Quarterly testing for serology, parasitology and bacteriology; specific pathogen free (SPF) conditions

Supplier	Inbred mice were obtained from The Jackson Laboratory (JAXÂ®, Bar Harbor, Maine). Collaborative Cross (CC) mice bred and obtained from Oak Ridge National Laboratory (Oak Ridge, TN 37831).
Ages	variable age
Feed	Irradiated Purina 5053: 5% fat, 20% protein given ad libitum
Water	Chlorinated 3â5 p.p.m. given ad libitum; Automatic: Edstrom
Housing	Optimice Single (75 sq. in.), Thoren Single (75 sq. in.), Thoren Duplex (52 sq. in.). All polycarbonate; Thoren filter top; Nestlets, igloos, PVC pipes environmental enrichment
Bedding	Harlan Softcob bedding
Photoperiod	14:10 h L:D; light intensities maintained at 3 lux at a distance of 30 in from the floor surface
Humidity	30-70%
Temperature	21 +/- 1.0°C
Health status	Quarterly testing for serology, parasitology and bacteriology; specific pathogen free (SPF) conditions