Chesler1 animal documentation

High-throughput pain-related behavioral phenotyping in 61 BXD recombinant inbred strains of mice   (2010)

Chesler EJ, Philip VM, Lariviere WR
With: Spence JS

See also: Chesler1 protocol


Chesler1_Animal

Acclimation and testing periods

Supplier
Both sexes of 61 BXD RI lines were studied. BXD 1–42/TyJ strains were obtained from the Jackson Laboratory (Bar Harbor, ME, USA). BXD RI lines, designated */Rww, were provided by Drs. Lu Lu and Robert W. Williams (University of Tennessee Health Science Center, Memphis TN), prior to importation at JAX. */Rww strains have been updated with current nomenclature (*/RwwJ) to best integrate these data with other MPD data.
Ages 8-9 wks of age
Feed
Irradiated Purina 5053: 5% fat, 20% protein given ad libitum
Water
Chlorinated 3–5 p.p.m. given ad libitum ; Automatic: Edstrom
Housing
Optimice Single (75 sq. in.), Thoren Single (75 sq. in.), Thoren Duplex (52 sq. in.). All polycarbonate; Optimice polycarbonate top, Thoren filter top; Nestlets, igloos, PVC pipes environmental enrichment
Bedding
Harlan Softcob bedding
Photoperiod
14:10 hr L:D; lights on at 0600 am and off at 2000 pm; lit with fluorescent ceiling lights
Humidity 30-70%
Temperature
21 +/- 1.0°C
Health status
Quarterly testing for serology, parasitology and bacteriology; specific pathogen free (SPF) conditions
BXD Strains Supplied by ORNL*
BXD43/Rww
BXD73/Rww
BXD44/Rww
BXD71/Rww
BXD45/Rww
BXD75/Rww
BXD48/Rww
BXD77/Rww
BXD50/Rww
BXD80/Rww
BXD51/Rww
BXD83/Rww
BXD55/Rww
BXD84/Rww
BXD56/Rww
BXD85/Rww
BXD60/Rww
BXD86/Rww
BXD61/Rww
BXD87/Rww
BXD62/Rww
BXD89/Rww
BXD63/Rww
BXD92/Rww
BXD65/Rww
BXD96/Rww
BXD66/Rww
BXD97/Rww
BXD67/Rww
BXD98/Rww
BXD68/Rww
BXD99/Rww
BXD69/Rww
BXD100/Rww
BXD70/Rww
 
BXD Strains Supplied by JAX®Mice
BXD1/TyJ BXD24/TyJ-Cep290rd16/J
BXD6/TyJ BXD27/TyJ
BXD8/TyJ BXD28/TyJ
BXD11/TyJ BXD29-Tlr4lps-2J/J
BXD12/TyJ BXD31/TyJ
BXD13/TyJ BXD32/TyJ
BXD14/TyJ BXD33/TyJ
BXD15/TyJ BXD34/TyJ
BXD16/TyJ BXD36/TyJ
BXD18/TyJ BXD38/TyJ
BXD19/TyJ BXD39/TyJ
BXD20/TyJ BXD40/TyJ
BXD21/TyJ BXD42/TyJ


Note: The strains designated as /RwwJ were originally named /Rww and provided for this study by Rob Williams prior to JAX importation and distribution.