CGDpheno3 animal documentation

Multi-system survey of mouse physiology in 8 inbred founder strains and 54 F1 hybrids of the Collaborative Cross   (2012)

Center for Genome Dynamics (CGD)
With: Svenson KL, Lenarcic AB, Churchill GA, Valdar W

See also: CGDpheno3 protocol


CGDpheno3 Animal Documentation

Acclimation & testing periods

Vendor
Mice were obtained from The Jackson Laboratory JAX®
Ages
Mice were 7-16 wks of age during testing
Feed
Free access to standard laboratory chow containing 6% fat by weight (LabDiet 5K52)
Water
Acidified water was given ad libitum
Housing
Same-sex mice were housed 2-5 per pen in duplex polycarbonate cages (31 x 31 x 214 cm) equipped with pressurized individually ventilated mouse racks (Thoren Caging Systems Inc.) with HEPA filtered air supply and exhaust
Room temperature
21-23°C ambient temperature
Humidity
~50%
Bedding
Steam autoclaved white pine shavings
Photoperiod
Mice were on a 12-h:12-h light:dark cycle beginning at 0600 h
Health status
Colonies were regularly monitored for viruses, bacterial species, ecto- and endo- parasites. All animal protocols were approved by the Jackson Laboratory Animal Care and Use Committee. Mouse handling and care complied with the Public Health Service animal welfare policy.