Acclimation & testing periods
Vendor Mice were born, raised, and maintained at The Jackson Laboratory JAX® - 10 males and 10 females for 71 inbred mouse strains Acclimation At 6-8 weeks of age, mice were transferred from production rooms to one of two animal rooms depending on their health status, designated as A and B for this study. Room A was at a lower health status than room B, due to the detection of Pneumocystis carinii, Pasturella pneumotropica, and Helicobacter spp. in room A, which were not detected in room B. Ages Mice were 10-13 wks of age on the day of sacrifice Feed Food pellets containing 6% fat (Lab diet 5K52, PMI Nutritional International, Bentwood, Mo) were provided ad libitum Water Acidified water was give ad libitum Housing Same-sex mice were housed 4 per pen in duplex polycarbonate cages (31x31x214cm) equipped with pressurized individually ventilated mouse racks (Thoren Caging Systems Inc.) with HEPA filtered air supply and exhaust Room temperature 21-23°C ambient temperature Humidity ~50% Bedding Steam autoclaved white pine shavings Photoperiod 10:14 (room A) or 12:12 (room B) light:dark cycle Health status Colonies were regularly monitored for viruses, bacterial species, ecto- and endo- parasites. All animal protocols were approved by the Jackson Laboratory Animal Care and Use Committee. Mouse handling and care complied with the Public Health Service animal welfare policy.