Bubier3 project protocol

Drug study: Opioid induced respiratory depression in 8 inbred founder strains of the Collaborative Cross   (2020)

Bubier JA, Chesler EJ
With: He H, Philip V, Donohue KD, O'Hara B

Project protocol - Contents


General Information for All Procedures: This protocol addresses the routine procedures used for conducting the morphine lethality study with the Piezo Sleep system.

Procedure 1: Morphine lethality assessment

Procedure PDF:

Definitions & Abbreviations: PVDF (polyvinylidene fluoride): sensor pads for bottom of each cage; USB DAQ: universal serial bus data acquisition module

Equipment, software, and supplies

  • Computer
  • National Instruments (NI) box
  • Polycarbonate cage wall units, with 302 stainless steel feeding grates
  • Acetate floors
  • PVDF sensor mats
  • Water bottles, with long sippers (150 mL)
  • Scale, 0.1 g accuracy
  • Pine shavings
  • Alpha-dri
  • Paper towels
  • HOBO Environment Tracker
  • Forceps

Reagents and solutions

  • Morphine (NIH NIDA Drug Supply)
  • Haemo-sol
  • Ethanol (70%)


  1. None


Primary References

Bubier JA, He H, Philip VM, Roy T, Hernandez CM, Bernat R, Donohue KD, O'Hara BF, Chesler EJ. Genetic variation regulates opioid-induced respiratory depression in mice. Sci Rep. 2020 Sep 11;10(1):14970. doi: 10.1038/s41598-020-71804-2.   PubMed 32917924     FullText