General Information for All Procedures: This protocol addresses the routine procedures used for conducting the morphine lethality study with the Piezo Sleep system.
Procedure 1: Morphine lethality assessment
Procedure PDF:
Definitions & Abbreviations: PVDF (polyvinylidene fluoride): sensor pads for bottom of each cage; USB DAQ: universal serial bus data acquisition module
Equipment, software, and supplies
- Computer
- National Instruments (NI) box
- Polycarbonate cage wall units, with 302 stainless steel feeding grates
- Acetate floors
- PVDF sensor mats
- Water bottles, with long sippers (150 mL)
- Scale, 0.1 g accuracy
- Pine shavings
- Alpha-dri
- Paper towels
- HOBO Environment Tracker
- Forceps
Reagents and solutions
- Morphine (NIH NIDA Drug Supply)
- Haemo-sol
- Ethanol (70%)
Steps
- None
Primary References
Bubier JA, He H, Philip VM, Roy T, Hernandez CM, Bernat R, Donohue KD, O'Hara BF, Chesler EJ. Genetic variation regulates opioid-induced respiratory depression in mice. Sci Rep. 2020 Sep 11;10(1):14970. doi: 10.1038/s41598-020-71804-2.
PubMed 32917924 FullText