Bubier3: Drug study: Opioid induced respiratory depression in 8 inbred founder strains of the Collaborative Cross (2020)

Bubier JA, He H, Philip VM, Roy T, Hernandez CM, Bernat R, Donohue KD, O'Hara BF, Chesler EJ. Genetic variation regulates opioid-induced respiratory depression in mice. Sci Rep. 2020 Sep 11;10(1):14970. doi: 10.1038/s41598-020-71804-2.   PubMed 32917924     FullText

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Investigators Jason A Bubier       The Jackson Laboratory,  Bar Harbor, ME
Elissa J Chesler       The Jackson Laboratory,  Bar Harbor, ME
Participants He H, Philip V, Donohue KD, O'Hara B
ContactJason A Bubier     jason.bubier@jax.org
Affiliated CenterCenter for Systems Neurogenetics of Addiction (CSNA)
AcknowledgementsNIH NIDA Drug Supply Program
Funding Provided ByNIH DA037927, AA018776, DA039841, DA048890, HL142489
Project type Phenotype strain survey data set
MPD identifiersBubier3     MPD:649
Data changelog No updates/corrections.       Initial release date: 12/2020.
Formatted citation
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This study measured respiratory phenotypes using signals obtained from a piezo electric sleep monitoring system in the eight founder strains of the Collaborative Cross and Diversity Outbred populations. This system offers a non-invasive, high-throughput technology to study respiratory depression in response to opioids in mice in a home-cage setting. The piezo technology was adapted to estimate average respiratory rates over specified time intervals for characterizing patterns associated with respiratory depression. This allows for the determination of respiratory depression and time to recovery or cessation of respiration.

Experimental groups in this study:
• Morphine    

Procedures conducted:
• monitoring system  Induced respiratory depression, time to recovery (for mice that survived), survival data (for mice that did not survive).

Mice: inbred w/CC8   8 strains   ♀♂   age 8-24wks   1 experimental group