Source of Mice: The Jackson Laboratory
Age (wks): 6-8 wks at shipping
Breeding: Some strains were bred in the JAX animal research facility.
Test Facility: The Jackson Laboratory, G3B, SPF Intermediate Level L
Feed: Sterilized 6% fat 5K52 chow, ad libitum (LabDiet/PMI Nutrition, St. Louis MO)
Water: Acidified (pH2-5.3) with vitamin K, ad libitum
Bedding: Pine shavings
Cage Type, Distributor, Material, Size: Plastic duplex pressurized individually vented Thoren cage #3 (30.80 x 30.80 x 14.5 cm). The ventilated caging provided a minimum of 60 air changes per hour within each cage.
Husbandry: Bedding changed weekly
Cage Density: 1-5
Cage Enrichment: Nestlet and shepard shack
Temperature (°C): 18-23°C
Humidity (%): 40-60%
Light / Dark Cycle: 12:12h
Care and Use Statement: All animal protocols were reviewed and approved by The Jackson Laboratory Institutional Animal Care and Use Committee.
Subject Identification: Ear notch