Project
protocol
-
Contents
Workflow
and
sampling
Step |
Procedure |
Equipment |
Data
collected |
1 |
Study 1: 20 min intra-strain test designed to elicit all types of social interactions (between resident and intruder) |
Clear polypropylene cage equipped with video camera |
Social behaviors |
| 2 |
Study 2: 30 min intra-strain test designed to reduce the number of aggressive behaviors (both mice put in cage simultaneously)
|
Clear polypropylene cage equipped with video camera |
Social behaviors |
Equipment
and
supplies
- Test chamber: Clear polypropylene cage (27.5 x 15.5 x 13.0 cm) with bedding covering the bottom
- Video camera: Panasonic VHS
Procedure: Study 1: Interaction for resident/intruder pairs (cohort 1)
- One male mouse is placed in the cage and allowed to explore freely for 15 min (resident).
- A second male mouse of the same strain is added to the cage (intruder).
- Behavior is recorded for 20 min.
- Videotape is reviewed by a naive observer and behavior scored for each pair of mice.
- Note: At the end of a session, both mice are removed from the chamber, and the chamber is cleaned and new bedding added before the next pair of mice is tested.
Procedure: Study 2: Interaction for simultaneously added pairs (cohort 2)
- Both mice of the same strain are added to the chamber simultaneously.
- Behavior is recorded for 30 min.
- Videotape is reviewed by a naive observer and behavior scored for each pair of mice.
- Note: At the end of a session, both mice are removed from the chamber, and the chamber is cleaned and new bedding added before the next pair of mice is tested.
Data
collected
by
investigator
- Study 1
- Mounting
- Wrestling
- *Allogrooming (not observed for any pair of mice)
- *Barbering (not observed for any pair of mice)
- *Huddling (not observed for any pair of mice)
- Following
- *Butt sniffing (observed for all pairs of mice)
- *Face sniffing (observed for all pairs of mice)
- Total time spent in social behavior
- Study 2
- Number of sniffs
- Time spent sniffing
- Number of negative social interactions
- Time spent engaged in negative social interactions
- Number of positive social interactions
- Time spent engaged in positive social interactions
- *Number of mounts (one 129S1/SvImJ mouse was observed mounting)
- Time spent engaged in mounting behavior
- Time engaged in any type of social behavior
Measures marked with * were the same for all mice tested except where noted. These measures were not accessioned. All submitted data are available in the download file.
Percentages computed by MPD |