Acclimation and testing periods
Ages Males 12 wks Feed Initially, each animal was provided with a pre-weighed ration (~20 g) of Purina Mouse chow (5.3 kcal/g) Water Two calibrated and preweighed (100 mL capacity, 0.1 g = 0.1 mL, Lab Products, Seaford, DE) sipper tubes each filled with water (~40 mL each)Bedding Wood shavings HousingAll mice were individually housed in plastic "tub" cages (30 cm x 20 cm x 15 cm) with a stainless steel grid lid Photoperiod 12-h light/12-h dark cycle with lights off at 2000 h Temperature 22 °C Health status All procedures were approved by the Queens College Institutional Animal Care and Use Committee