MPD: Berndt1: animal documentation

Berndt1 animal documentation

Methacholine-induced changes in airway resistance in 37 inbred strains of mice (2010)

Berndt A, Leme AS, Paigen B, Shapiro SD

Berndt1_Animal

Acclimation and testing periods

Supplier All mice were raised and maintained in colonies at The Jackson Laboratory (JAX®, Bar Harbor, ME) until 6-11 wks of age

Ages

Phenotypic analysis conducted at 9-12 wks of age

Feed

Chow diet (LabDiet® JR Rat and Mouse/Auto 6F 5K52) given ad libitum

Water

Ad libitum

Housing
4-5 mice per cage, separated by sex and strain in clear polycarbonate/ polysulfone cages (28 x 18 x 13 cm)

Bedding

Pine shaving bedding and topped with a polyester filter

Photoperiod

12 h light/12 h dark cycle

Temperature

22 ± 1.0°C

Health status

Regularly monitored for (and found to be free of) 15 viruses, 17 bacterial species, Mycoplasma spp., and parasites. All animal protocols were reviewed and approved by the Animal Care and Use Committees at the University of Pittsburgh and The Jackson Laboratory.

Supplier	All mice were raised and maintained in colonies at The Jackson Laboratory (JAX®, Bar Harbor, ME) until 6-11 wks of age
Ages	Phenotypic analysis conducted at 9-12 wks of age
Feed	Chow diet (LabDiet® JR Rat and Mouse/Auto 6F 5K52) given ad libitum
Water	Ad libitum
Housing	4-5 mice per cage, separated by sex and strain in clear polycarbonate/ polysulfone cages (28 x 18 x 13 cm)
Bedding	Pine shaving bedding and topped with a polyester filter
Photoperiod	12 h light/12 h dark cycle
Temperature	22 ± 1.0°C
Health status	Regularly monitored for (and found to be free of) 15 viruses, 17 bacterial species, Mycoplasma spp., and parasites. All animal protocols were reviewed and approved by the Animal Care and Use Committees at the University of Pittsburgh and The Jackson Laboratory.