Berndt1 animal documentation

Methacholine-induced changes in airway resistance in 37 inbred strains of mice   (2010)

Berndt A, Leme AS, Paigen B, Shapiro SD

See also: Berndt1 protocol


Berndt1_Animal

Acclimation and testing periods

Supplier All mice were raised and maintained in colonies at The Jackson Laboratory (JAX®, Bar Harbor, ME) until 6-11 wks of age
Ages
Phenotypic analysis conducted at 9-12 wks of age
Feed
Chow diet (LabDiet® JR Rat and Mouse/Auto 6F 5K52) given ad libitum
Water
Ad libitum
Housing
4-5 mice per cage, separated by sex and strain in clear polycarbonate/ polysulfone cages (28 x 18 x 13 cm)
Bedding
Pine shaving bedding and topped with a polyester filter
Photoperiod
12 h light/12 h dark cycle
Temperature
22 ± 1.0°C
Health status
Regularly monitored for (and found to be free of) 15 viruses, 17 bacterial species, Mycoplasma spp., and parasites, as described. All animal protocols were reviewed and approved by the Animal Care and Use Committees at the University of Pittsburgh and The Jackson Laboratory.