Berndt1 animal documentation

Methacholine-induced changes in airway resistance in 37 inbred strains of mice   (2010)

Berndt A, Leme AS, Paigen B, Shapiro SD

See also: Berndt1 protocol


Acclimation and testing periods

Supplier All mice were raised and maintained in colonies at The Jackson Laboratory (JAX®, Bar Harbor, ME) until 6-11 wks of age
Phenotypic analysis conducted at 9-12 wks of age
Chow diet (LabDiet® JR Rat and Mouse/Auto 6F 5K52) given ad libitum
Ad libitum
4-5 mice per cage, separated by sex and strain in clear polycarbonate/ polysulfone cages (28 x 18 x 13 cm)
Pine shaving bedding and topped with a polyester filter
12 h light/12 h dark cycle
22 ± 1.0°C
Health status
Regularly monitored for (and found to be free of) 15 viruses, 17 bacterial species, Mycoplasma spp., and parasites, as described. All animal protocols were reviewed and approved by the Animal Care and Use Committees at the University of Pittsburgh and The Jackson Laboratory.