Acclimation and testing periods
Supplier All breeding and testing of live mice* were conducted in the Specific Pathogen Free (SPF) facility at the Institute for Behavioral Genetics, University of Colorado at Boulder. Acclimation 1-2 wks before testing Ages 8-14 wks of age at time of testing Feed Sterilized rodent diet by Teklad (4% fat, 24% protein) given ad libitum Water Given ad libitum Housing Mice of the same strain and sex were housed together at weaning, ~21 to 25 days of age; no singly housed mice were tested Bedding Harlan Softcob bedding Photoperiod 12:12 hr L:D; lights on at 0700; lit with fluorescent ceiling lights Humidity 12% relative Temperature 22 ± 1 °C Regulatory compliance All procedures were approved by the University of Colorado Institutional Animal Care and Use Committee, in accordance with National Institute of Health guidelines.
*The ILSXISS#/Tej (formerly LXS) recombinant inbred lines were generated from crosses between ILS/Ibg (Inbred Long Sleep) and ISS/Ibg (Inbred Short Sleep). These two progenitor strains were developed from the LS and SS selected lines developed from a heterogenous stock derived from an 8 way cross (A, AKR, BALB/c, C3H/2, C57BL, DBA/2, IS/Bi and RIII). ILS and ISS are the product of inbreeding LS and SS, lines selected for long or short duration of loss-of-righting response following high-dose intraperitoneal administration of ethanol. To produce RI strains, ILS and ISS mice were intercrossed and 4 reciprocal matings of F1 mice were used to produce the F2 generation. Each of the four F2 crosses generated a unique combination of Y Chromosome and maternal (mitochondrial) contributions. Breedings were conducted in the Specific Pathogen Free (SPF) facility at the Institute for Behavioral Genetics, University of Colorado at Boulder (see Bennett et. al., 2005).
Note: Strain names for mice tested prior to importation to The Jackson Laboratory have been updated using current strain nomenclature and assigned JAX vendor codes (if available) in order to facilitate comparisons across projects.