Attie2 project protocol

Diet effects on Type 2 diabetes associated physiological traits in a Diversity Outbred (DO) mouse population on a high-fat high-sucrose diet   (2018)

Keller MP, Churchill GA, Attie AD
With: Gatti DM, Schueler KL, Rabaglia ME, Stapleton DS, Simecek P, Vincent M, Allen S, Broman AT, Bacher R, Kendziorski C, Broman KW, Yandell BS




Project protocol - Contents

Workflow and sampling

Step Description Equipment Data collected
1 Administer HFHS diet at 4 wks of age - -
2 Mice weighed weekly, food consumption measured Balance Body weight and food consumption
3 Blood chemistry at 6, 10, 14 wks and at sac (4h fast) after HFHS diet - Plasma glucose, insulin, triglyceride
4 Oral glucose tolerance test (4h fast) after ~14 wks on HFHS diet Kits Plasma glucose and insulin at 0, 5, 15, 30, 60, 120 min
5 Blood chemistry after ~14 wks on HFHS diet - Plasma adiponectin and leptin
6 Mice euthanized and islets harvested Stereomicroscope Number of islets per mouse
7 Ex vivo insulin and glucagon secretion in response to various amounts of glucose and other compounds Kits Insulin and glucagon secretion
8 BMD, organ weights DXA, balance Tibia BMD, heart weight, liver weight, gonadal fat pad weight
9 Bile acids profiled from feces - Quantity of specific bile acids per mg feces

References

Primary References

Keller MP, Gatti DM, Schueler KL, Rabaglia ME, Stapleton DS, Simecek P, Vincent M, Allen S, Broman AT, Bacher R, Kendziorski C, Broman KW, Yandell BS, Churchill GA, Attie AD. Genetic Drivers of Pancreatic Islet Function. Genetics. 2018 May;209(1):335-356. doi: 10.1534/genetics.118.300864. Epub 2018 Mar 22.   PubMed 29567659     FullText