MPD: Data set: Attie2

Attie2: Diet effects on Type 2 diabetes associated physiological traits in a Diversity Outbred (DO) mouse population on a high-fat high-sucrose diet (2018)

Keller MP, Gatti DM, Schueler KL, Rabaglia ME, Stapleton DS, Simecek P, Vincent M, Allen S, Broman AT, Bacher R, Kendziorski C, Broman KW, Yandell BS, Churchill GA, Attie AD. Genetic Drivers of Pancreatic Islet Function. Genetics. 2018 May;209(1):335-356. doi: 10.1534/genetics.118.300864. Epub 2018 Mar 22. PubMed 29567659 FullText

Diversity Outbred mice were metabolically challenged with a high-fat (45% kcal), high-sucrose (34%) diet from 4 wks of age. DO mice were assessed for physiological traits related to Type 2 Diabetes at various time points. Pancreatic islet gene expression data and genotypes are available at DODB (search for Attie).

Experimental groups in this study:
• High-fat high-sucrose diet

Procedures conducted:

• colony observation		Number of days on high-fat high-sucrose diet.
• body weight		Weekly.
• intake monitoring		Food intake. Weekly.
• metabolic panel		Glucose, HOMA-IR, HOMA-B.
• hormone quantification		Plasma insulin, adiponectin, leptin. Ex vivo insulin secretion.
• lipid profile		Triglyceride.
• glucose tolerance		Area under curve.
• microscopy		Number of islets harvested.
• DXA		Bone mineral density. Isolated tibia.
• organ weights		Heart, liver, gonadal fat pads.
• biomarker quantification		Bile acids in feces.

Attie2 downloads
• Download Attie2 project data set animal data, as uploaded
• Download Attie2 animal data matrix with factor-related expansion applied as necessary
• Download Attie2 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Attie2 measures:

MA:0000059 blood
MA:0000072 heart
MA:0000120 pancreas
MA:0000127 pancreatic islet
MA:0000358 liver
MA:0001361 tibia
MA:0002501 plasma
MA:0002509 feces
MA:0002547 gonadal fat pad
MP:0000187 abnormal triglyceride level
MP:0000188 abnormal circulating glucose level
MP:0001259 abnormal body weight
MP:0001560 abnormal circulating insulin level
MP:0003564 abnormal insulin secretion
MP:0003565 abnormal glucagon secretion
MP:0003868 abnormal feces composition
MP:0004773 abnormal bile composition
MP:0004847 abnormal liver weight
MP:0004857 abnormal heart weight
MP:0005291 abnormal glucose tolerance
MP:0005335 abnormal gonadal fat pad morphology
MP:0005449 abnormal food intake
MP:0005667 abnormal circulating leptin level
MP:0009166 abnormal pancreatic islet number
MP:0010119 abnormal bone mineral density
MP:0030967 abnormal circulating adiponectin level
VT:0000187 triglyceride amount
VT:0000188 blood glucose amount
VT:0001259 body mass
VT:0001560 blood insulin amount
VT:0001944 pancreas morphology trait
VT:0002069 consumption behavior trait
VT:0003402 liver mass
VT:0003564 insulin secretion trait
VT:0003565 glucagon secretion trait
VT:0005007 bone mineral mass
VT:0005667 blood leptin amount
VT:0007028 heart mass
VT:0010423 gonadal fat pad mass
VT:0010470 pancreas insulin amount
VT:0010545 blood adiponectin amount
VT:0010919 feces bile acid amount
VT:0015088 glucose metabolism trait

Investigators	Mark P Keller University of Wisconsin, Madison, WI Gary A Churchill The Jackson Laboratory, Bar Harbor, ME Alan D Attie University of Wisconsin, Madison, WI
Participants	Gatti DM, Schueler KL, Rabaglia ME, Stapleton DS, Simecek P, Vincent M, Allen S, Broman AT, Bacher R, Kendziorski C, Broman KW, Yandell BS
Contact	Alan D Attie attie@biochem.wisc.edu Lab web site
Funding Provided By	NIH DK101573, DK102948, GM102756, AI117924, GM076483; Clinical and Translational Science Award program, through the NIH National Center for Advancing Translational Sciences (grant UL1 TR-000427)
Project type	Phenotype strain survey data set
MPD identifiers	Attie2 MPD:1020
Data changelog	No updates/corrections. Initial release date: 10/2020.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice: DO population ♀♂ age 4-26wks 1 experimental group

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	Sex	Age	Sample Size (Avg)
Attie2:14	Attie2	colony observation	high-fat high-sucrose diet	number of days on diet [n] diet_days 110631	DO population	both	4-26wks	N=242

Attie2:15	Attie2	body weight	high-fat high-sucrose diet	body weight after diet [g] bw 110632 wk: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 (5 more)...	DO population	both	5-26wks	N=250

Attie2:37	Attie2	body weight	high-fat high-sucrose diet	change in body weight after diet (compared to wk 1) [g] bw_chg 110654 wk: 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17	DO population	both	6-21wks	N=250

Attie2:53	Attie2	body weight	high-fat high-sucrose diet	change in body weight after diet (compared to wk 10), wk 17 [g] bw_chg_wk17v10 110670	DO population	both	21wks	N=236

Attie2:54	Attie2	intake monitoring	high-fat high-sucrose diet	food consumption after diet [g] food 110671 wk: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 (4 more)...	DO population	both	5-25wks	N=250

Attie2:75	Attie2	intake monitoring	high-fat high-sucrose diet	food consumption average after diet (1 to 21 wks) [g] food_ave 110692	DO population	both	5-25wks	N=250

Attie2:76	Attie2	metabolic panel	high-fat high-sucrose diet	plasma glucose after diet (4h fast) [mg/dL] GLU 110693 wk: 6 10 14 22	DO population	both	10-26wks	N=250

Attie2:80	Attie2	hormone quantification	high-fat high-sucrose diet	plasma insulin after diet (4h fast) [ng/mL] INS 110697 wk: 6 10 14 22	DO population	both	10-26wks	N=250

Attie2:84	Attie2	lipid profile	high-fat high-sucrose diet	plasma triglyceride after diet (4h fast) [mg/dL] TG 110701 wk: 6 10 14 22	DO population	both	10-26wks	N=250

Attie2:88	Attie2	body weight	high-fat high-sucrose diet	body weight at time of oral glucose tolerance test (~14 wks on diet) [g] bw_oGTT 110705	DO population	both	18wks	N=248

Attie2:89	Attie2	glucose tolerance	high-fat high-sucrose diet	glucose level at t=0 after ~14 wks on diet (4h fast) [mg/dL] GLU_0min 110706	DO population	both	18wks	N=248

Attie2:90	Attie2	glucose tolerance	high-fat high-sucrose diet	insulin level at t=0 after ~14 wks on diet (4h fast) [ng/mL] INS_0min 110707	DO population	both	18wks	N=248

Attie2:91	Attie2	glucose tolerance	high-fat high-sucrose diet	HOMA-IR: (glucose x insulin) / 405 at t=0 after ~14 wks on diet (4h fast) HOMA_IR 110708	DO population	both	18wks	N=248

Attie2:92	Attie2	glucose tolerance	high-fat high-sucrose diet	HOMA-B: (360 x insulin) / (glucose minus 63) at t=0 after ~14 wks on diet (4h fast) HOMA_B 110709	DO population	both	18wks	N=246

Attie2:93	Attie2	glucose tolerance	high-fat high-sucrose diet	glucose area under curve (AUC) after ~14 wks on diet without correction for baseline difference (4h fast) [AUC] GLU_AUC_tot 110710	DO population	both	18wks	N=248

Attie2:94	Attie2	glucose tolerance	high-fat high-sucrose diet	glucose area under curve (AUC) after ~14 wks on diet after subtraction of glucose at t=0 (4h fast) [AUC] GLU_AUC_adj 110711	DO population	both	18wks	N=248

Attie2:95	Attie2	glucose tolerance	high-fat high-sucrose diet	insulin area under curve (AUC) after ~14 wks on diet without correction for baseline difference (4h fast) [AUC] INS_AUC_tot 110712	DO population	both	18wks	N=248

Attie2:96	Attie2	glucose tolerance	high-fat high-sucrose diet	insulin area under curve (AUC) after ~14 wks on diet after subtraction of insulin at t=0 (4h fast) [AUC] INS_AUC_adj 110713	DO population	both	18wks	N=248

Attie2:97	Attie2	hormone quantification	high-fat high-sucrose diet	plasma adiponectin measured at the time oral glucose tolerance test performed after ~14 wks on diet (4h fast) [µg/mL] adiponectin 110714	DO population	both	18wks	N=144

Attie2:98	Attie2	hormone quantification	high-fat high-sucrose diet	plasma leptin measured at the time oral glucose tolerance test performed after ~14 wks on diet (4h fast) [ng/mL] leptin 110715	DO population	both	18wks	N=96

Attie2:99	Attie2	microscopy	high-fat high-sucrose diet	number of islets harvested per mouse after diet [n] islets 110716	DO population	both	19-26wks	N=242

Attie2:100	Attie2	hormone quantification	high-fat high-sucrose diet	amount of insulin per islet after diet [ng/mL] INS_per_islet 110717	DO population	both	19-26wks	N=241

Attie2:101	Attie2	hormone quantification	high-fat high-sucrose diet	whole pancreas insulin content after diet (insulin content per islet times number of islets) [ng/mL] INS_per_pancreas 110718	DO population	both	19-26wks	N=241

Attie2:102	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to glucose [ng/mL] INS_GLU 110719 3.3 mM 8.3 mM 16.7 mM	DO population	both	19-26wks	N=242

Attie2:105	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 3.3 mM glucose plus 40 mM KCl [ng/mL] INS_GLU3p3_KCl 110722	DO population	both	19-26wks	N=240

Attie2:106	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus 100 nM GLP-1 [ng/mL] INS_GLU8p3_GLP1 110723	DO population	both	19-26wks	N=240

Attie2:107	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus a mix of amino acids (Ala, Glu, Leu) [ng/mL] INS_GLU8p3_AA 110724	DO population	both	19-26wks	N=240

Attie2:108	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 16.7 mM glucose plus 0.5 mM palmitic acid [ng/mL] INS_GLU16p7_PA 110725	DO population	both	19-26wks	N=240

Attie2:109	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose, expressed as fold-change over that observed with 3.3 mM glucose [ratio] INS_GLU8p3_fc_G3p3 110726	DO population	both	19-26wks	N=240

Attie2:110	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 16.7 mM glucose, expressed as fold-change over that observed with 3.3.mM glucose [ratio] INS_GLU16p7_fc_G3p3 110727	DO population	both	19-26wks	N=240

Attie2:111	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 3.3 mM glucose plus 40 mM KCl, expressed as fold-change over that observed with 3.3 mM glucose [ratio] INS_GLU3p3__KCl_fc_G3p3 110728	DO population	both	19-26wks	N=240

Attie2:112	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus 100 nM GLP-1, expressed as fold-change over that observed with 3.3 mM glucose [ratio] INS_GLU8p3_GLP1_fc_G3p3 110729	DO population	both	19-26wks	N=240

Attie2:113	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus a mix of amino acids (Ala, Glu, Leu), expressed as fold-change over that observed with 3.3 mM glucose [ratio] INS_GLU8p3_AA_fc_G3p3 110730	DO population	both	19-26wks	N=240

Attie2:114	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 16.7 mM glucose plus palmitic acid, expressed as fold-change over that observed with 3.3 mM glucose [ratio] INS_GLU16p7_PA_fc_G3p3 110731	DO population	both	19-26wks	N=240

Attie2:115	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 3.3 mM glucose plus 40 mM KCl, expressed as fold-change over that observed with 8.3 mM glucose [ratio] INS_GLU8p3_GLP1_fc_G8p3 110732	DO population	both	19-26wks	N=240

Attie2:116	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus a mix of amino acids (Ala, Glu, Leu), expressed as fold-change over that observed with 8.3 mM glucose [ratio] INS_GLU8p3_AA_fc_G8p3 110733	DO population	both	19-26wks	N=240

Attie2:117	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 16.7 mM glucose plus 0.5 mM palmitic acid, expressed as fold-change over that observed with 16.7 mM glucose [ratio] INS_GLU16p7_PA_fc_G16p7 110734	DO population	both	19-26wks	N=240

Attie2:118	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to glucose, expressed as a percentage of total insulin per mouse [%] INS_pct_GLU 110735 3.3 mM 8.3 mM 16.7 mM	DO population	both	19-26wks	N=241

Attie2:121	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 3.3 mM glucose plus 40 mM KCl, expressed as a percentage of total insulin per mouse [%] INS_GLU3p3_KCl_pct 110738	DO population	both	19-26wks	N=239

Attie2:122	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus 100 nM GLP-1, expressed as a percentage of total insulin per mouse [%] INS_GLU8p3_GLP1_pct 110739	DO population	both	19-26wks	N=239

Attie2:123	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus a mix of amino acids (Ala, Glu, Leu), expressed as a percentage of total insulin per mouse [%] INS_GLU8p3_AA_pct 110740	DO population	both	19-26wks	N=239

Attie2:124	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 16.7 mM glucose plus 0.5 mM palmitic acid, expressed as a percentage of total insulin per mouse [%] INS_GLU16p7_PA_pct 110741	DO population	both	19-26wks	N=239

Attie2:125	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to glucose (geometric mean) [ng/mL] INS_gm_GLU 110742 3.3 mM 8.3 mM 16.7 mM	DO population	both	19-26wks	N=242

Attie2:128	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 3.3 mM glucose plus 40 mM KCl (geometric mean) [ng/mL] INS_gm_GLU3p3_KCl 110745	DO population	both	19-26wks	N=240

Attie2:129	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus 100 nM GLP-1 (geometric mean) [ng/mL] INS_gm_GLU8p3_GLP1 110746	DO population	both	19-26wks	N=240

Attie2:130	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 8.3 mM glucose plus a mix of amino acids (Ala, Glu, Leu) (geometric mean) [ng/mL] INS_gm_GLU8p3_AA 110747	DO population	both	19-26wks	N=240

Attie2:131	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo insulin secretion after diet in response to 16.7 mM glucose plus 0.5 mM palmitic acid (geometric mean) [ng/mL] INS_gm_GLU16p7_PA 110748	DO population	both	19-26wks	N=240

Attie2:132	Attie2	hormone quantification	high-fat high-sucrose diet	amount of glucagon per islet after diet [ng/mL] GCG_per_islet 110749	DO population	both	19-26wks	N=241

Attie2:133	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo glucagon secretion after diet in response to 3.3 mM glucose plus 40 mM KCl [ng/mL] GCG_GLU3p3_KCl 110750	DO population	both	19-26wks	N=182

Attie2:134	Attie2	hormone quantification	high-fat high-sucrose diet	ex vivo glucagon secretion after diet in response to 3.3 mM glucose plus 40 mM KCl, expressed as a percentage of total glucagon per mouse [%] GCG_GLU3p3_KCl_pct 110751	DO population	both	19-26wks	N=182

Attie2:135	Attie2	DXA	high-fat high-sucrose diet	tibia bone mineral density (on isolated bone) after diet [g/cm²] BMD 110752	DO population	both	19-26wks	N=241

Attie2:136	Attie2	organ weights	high-fat high-sucrose diet	heart weight after diet [g] heart_wt 110753	DO population	both	19-26wks	N=194

Attie2:137	Attie2	organ weights	high-fat high-sucrose diet	liver left lobe weight after diet [g] liver_wt_L 110754	DO population	both	19-26wks	N=194

Attie2:138	Attie2	organ weights	high-fat high-sucrose diet	liver weight minus left lobe after diet [g] liver_wt_remain 110755	DO population	both	19-26wks	N=194

Attie2:139	Attie2	organ weights	high-fat high-sucrose diet	liver weight total after diet [g] liver_wt_tot 110756	DO population	both	19-26wks	N=194

Attie2:140	Attie2	organ weights	high-fat high-sucrose diet	gonadal fat pad weight after diet [g] gonadal_wt 110757 left right	DO population	both	19-26wks	N=194

Attie2:142	Attie2	organ weights	high-fat high-sucrose diet	gonadal fat pad weight after diet, total [g] gonadal_wt_tot 110759	DO population	both	19-26wks	N=194

Attie2:143	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid deoxycholate in feces after diet (per mg feces) [nmol/mg] DCA 110760	DO population	both	19-26wks	N=48

Attie2:144	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid alpha-muricholate in feces after diet (per mg feces) [nmol/mg] aMCA 110761	DO population	both	19-26wks	N=48

Attie2:145	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid beta-muricholate in feces after diet (per mg feces) [nmol/mg] bMCA 110762	DO population	both	19-26wks	N=48

Attie2:146	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid cholate in feces after diet (per mg feces) [nmol/mg] CA 110763	DO population	both	19-26wks	N=48

Attie2:147	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid glycolithocholate in feces after diet (per mg feces) [nmol/mg] GLiCA 110764	DO population	both	19-26wks	N=48

Attie2:148	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid glycochenodeoxycholate in feces after diet (per mg feces) [nmol/mg] GChDCA 110765	DO population	both	19-26wks	N=48

Attie2:149	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid glycoursodeoxycholate in feces after diet (per mg feces) [nmol/mg] GUDCA 110766	DO population	both	19-26wks	N=48

Attie2:150	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid glycodeoxycholate in feces after diet (per mg feces) [nmol/mg] GDCA 110767	DO population	both	19-26wks	N=48

Attie2:151	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid glycocholate in feces after diet (per mg feces) [nmol/mg] GCA 110768	DO population	both	19-26wks	N=48

Attie2:152	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid taurolithocholate in feces after diet (per mg feces) [nmol/mg] TLiCA 110769	DO population	both	19-26wks	N=47

Attie2:153	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid taurohyodeoxycholate in feces after diet (per mg feces) [nmol/mg] THDCA 110770	DO population	both	19-26wks	N=48

Attie2:154	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid tauroursodeoxycholate in feces after diet (per mg feces) [nmol/mg] TUDCA 110771	DO population	both	19-26wks	N=48

Attie2:155	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid taurochenodeoxycholate in feces after diet (per mg feces) [nmol/mg] TChDCA 110772	DO population	both	19-26wks	N=48

Attie2:156	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid taurodeoxycholate in feces after diet (per mg feces) [nmol/mg] TDCA 110773	DO population	both	19-26wks	N=48

Attie2:157	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid taurocholate in feces after diet (per mg feces) [nmol/mg] TCA 110774	DO population	both	19-26wks	N=48

Attie2:158	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid tauro-alpha-muricholate in feces after diet (per mg feces) [nmol/mg] TaMCA 110775	DO population	both	19-26wks	N=48

Attie2:159	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid tauro-beta-muricholate in feces after diet (per mg feces) [nmol/mg] TbMCA 110776	DO population	both	19-26wks	N=48

Attie2:160	Attie2	biomarker quantification	high-fat high-sucrose diet	bile acid chenodeoxycholate in feces after diet (per mg feces) [nmol/mg] ChDCA 110777	DO population	both	19-26wks	N=48

Attie2:161	Attie2	biomarker quantification	high-fat high-sucrose diet	total bile acid in feces after diet (per mg feces) [nmol/mg] bile_acid_total 110778	DO population	both	19-26wks	N=48

Attie2: Diet effects on Type 2 diabetes associated physiological traits in a Diversity Outbred (DO) mouse population on a high-fat high-sucrose diet (2018)

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