Attie2: Diet effects on Type 2 diabetes associated physiological traits in a Diversity Outbred (DO) mouse population on a high-fat high-sucrose diet (2018)

Keller MP, Gatti DM, Schueler KL, Rabaglia ME, Stapleton DS, Simecek P, Vincent M, Allen S, Broman AT, Bacher R, Kendziorski C, Broman KW, Yandell BS, Churchill GA, Attie AD. Genetic Drivers of Pancreatic Islet Function. Genetics. 2018 May;209(1):335-356. doi: 10.1534/genetics.118.300864. Epub 2018 Mar 22.   PubMed 29567659     FullText

Attie2 downloads
• Download Attie2 project data set     animal data, as uploaded
• Download Attie2 animal data matrix     with factor-related expansion applied as necessary
• Download Attie2 strain means, SD, N, etc.     one row per strain/sex/measure
Ontology terms mapped to Attie2 measures:
  MA:0000059   blood
  MA:0000072   heart
  MA:0000120   pancreas
  MA:0000127   pancreatic islet
  MA:0000358   liver
  MA:0001361   tibia
  MA:0002501   plasma
  MA:0002509   feces
  MA:0002547   gonadal fat pad
  MP:0000187   abnormal triglyceride level
  MP:0000188   abnormal circulating glucose level
  MP:0001259   abnormal body weight
  MP:0001560   abnormal circulating insulin level
  MP:0003564   abnormal insulin secretion
  MP:0003565   abnormal glucagon secretion
  MP:0003868   abnormal feces composition
  MP:0004773   abnormal bile composition
  MP:0004847   abnormal liver weight
  MP:0004857   abnormal heart weight
  MP:0005291   abnormal glucose tolerance
  MP:0005335   abnormal gonadal fat pad morphology
  MP:0005449   abnormal food intake
  MP:0005667   abnormal circulating leptin level
  MP:0009166   abnormal pancreatic islet number
  MP:0010119   abnormal bone mineral density
  MP:0030967   abnormal circulating adiponectin level
  VT:0000187   triglyceride amount
  VT:0000188   blood glucose amount
  VT:0001259   body mass
  VT:0001560   blood insulin amount
  VT:0001944   pancreas morphology trait
  VT:0002069   consumption behavior trait
  VT:0003402   liver mass
  VT:0003564   insulin secretion trait
  VT:0003565   glucagon secretion trait
  VT:0005007   bone mineral mass
  VT:0005667   blood leptin amount
  VT:0007028   heart mass
  VT:0010423   gonadal fat pad mass
  VT:0010470   pancreas insulin amount
  VT:0010545   blood adiponectin amount
  VT:0010919   feces bile acid amount
  VT:0015088   glucose metabolism trait

Investigators Mark P Keller       University of Wisconsin,  Madison, WI
Gary A Churchill       The Jackson Laboratory,  Bar Harbor, ME
Alan D Attie       University of Wisconsin,  Madison, WI
Participants Gatti DM, Schueler KL, Rabaglia ME, Stapleton DS, Simecek P, Vincent M, Allen S, Broman AT, Bacher R, Kendziorski C, Broman KW, Yandell BS
ContactAlan D Attie     Lab web site
Funding Provided ByNIH DK101573, DK102948, GM102756, AI117924, GM076483; Clinical and Translational Science Award program, through the NIH National Center for Advancing Translational Sciences (grant UL1 TR-000427)
Project type Phenotype strain survey data set
MPD identifiersAttie2     MPD:1020
Data changelog No updates/corrections.       Initial release date: 10/2020.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
Diversity Outbred mice were metabolically challenged with a high-fat (45% kcal), high-sucrose (34%) diet from 4 wks of age. DO mice were assessed for physiological traits related to Type 2 Diabetes at various time points. Pancreatic islet gene expression data and genotypes are available at DODB (search for Attie).

Experimental groups in this study:
• High-fat high-sucrose diet    

Procedures conducted:
• colony observation  Number of days on high-fat high-sucrose diet.
• body weight  Weekly.
• intake monitoring  Food intake. Weekly.
• metabolic panel  Glucose, HOMA-IR, HOMA-B.
• hormone quantification  Plasma insulin, adiponectin, leptin. Ex vivo insulin secretion.
• lipid profile  Triglyceride.
• glucose tolerance  Area under curve.
• microscopy  Number of islets harvested.
• DXA  Bone mineral density. Isolated tibia.
• organ weights  Heart, liver, gonadal fat pads.
• biomarker quantification  Bile acids in feces.

Mice: DO population   ♀♂   age 4-26wks   1 experimental group