Project measure / variable:   GMC20   IgG2a_21

ID, description, units MPD:56909   IgG2a_21   immunoglobulin G2a (plasma IgG2a)   [µg/mL]  at age 20-21wks  
Data set, strains GMC20   inbred w/CC8   8 strains     sex: both     age: 20-21wks
Procedure immunoglobulin quantification
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  STRAIN COMPARISON PLOT
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GMC20 - immunoglobulin G2a (plasma IgG2a) at age 20-21wks



  MEASURE SUMMARY
Measure Summary FemaleMale
Number of strains tested8 strains8 strains
Mean of the strain means294   µg/mL 345   µg/mL
Median of the strain means292   µg/mL 280   µg/mL
SD of the strain means± 167 ± 213
Coefficient of variation (CV)0.569 0.617
Min–max range of strain means112   –   603   µg/mL 179   –   820   µg/mL
Mean sample size per strain7.6   mice 8.8   mice


  ANOVA, Q-Q NORMALITY ASSESSMENT
ANOVA summary      
FactorDFSum of squaresMean sum of squaresF valuep value (Pr>F)
sex 1 100921.8116 100921.8116 1.0676 0.3036
strain 7 4605563.1988 657937.5998 6.9603 < 0.0001
sex:strain 7 315006.024 45000.8606 0.4761 0.8502
Residuals 115 10870632.9474 94527.243


Q-Q normality assessment based on residuals

  


  STRAIN MEANS (UNADJUSTED)
  
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Strain Sex Mean SD N mice SEM CV Min, Max Z score
129S1/SvImJ f 603.0 225.0   10 71.2 0.373 249.0, 1069.0 1.85
129S1/SvImJ m 820.0 565.0   12 163.0 0.69 115.0, 1593.0 2.23
A/J f 322.0 186.0   10 58.9 0.579 99.7, 597.0 0.17
A/J m 354.0 359.0   11 108.0 1.02 114.0, 1400.0 0.04
C57BL/6J f 430.0 568.0   9 189.0 1.32 38.5, 1609.0 0.82
C57BL/6J m 299.0 358.0   7 135.0 1.2 48.3, 1078.0 -0.21
CAST/EiJ f 112.0 57.4   6 23.5 0.515 47.7, 207.0 -1.09
CAST/EiJ m 180.0 66.7   7 25.2 0.371 112.0, 303.0 -0.77
NOD/ShiLtJ f 160.0 98.4   5 44.0 0.615 53.0, 301.0 -0.8
NOD/ShiLtJ m 179.0 59.7   6 24.4 0.333 123.0, 254.0 -0.78
NZO/HlLtJ f 332.0 165.0   7 62.4 0.498 153.0, 615.0 0.23
NZO/HlLtJ m 448.0 348.0   8 123.0 0.777 131.0, 1278.0 0.49
PWK/PhJ f 261.0 217.0   6 88.5 0.829 38.2, 556.0 -0.2
PWK/PhJ m 260.0 172.0   10 54.4 0.661 56.3, 504.0 -0.4
WSB/EiJ f 130.0 77.3   8 27.3 0.595 50.3, 309.0 -0.98
WSB/EiJ m 217.0 189.0   9 63.1 0.871 57.4, 654.0 -0.6


  LEAST SQUARES MEANS (MODEL-ADJUSTED)
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ f 603.002 97.2251 795.5863 410.4177
129S1/SvImJ m 819.515 88.754 995.3196 643.7104
A/J f 322.178 97.2251 514.7623 129.5937
A/J m 353.7636 92.7005 537.3855 170.1418
C57BL/6J f 429.7022 102.4843 632.7039 226.7006
C57BL/6J m 298.8386 116.2062 529.0208 68.6564
CAST/EiJ f 111.5333 125.5171 360.1585 -137.0919
CAST/EiJ m 179.6257 116.2062 409.8079 -50.5565
NOD/ShiLtJ f 160.046 137.4971 432.4013 -112.3093
NOD/ShiLtJ m 179.23 125.5171 427.8552 -69.3952
NZO/HlLtJ f 331.84 116.2062 562.0222 101.6578
NZO/HlLtJ m 447.9988 108.701 663.3145 232.683
PWK/PhJ f 261.4067 125.5171 510.0319 12.7815
PWK/PhJ m 260.272 97.2251 452.8563 67.6877
WSB/EiJ f 129.8187 108.701 345.1345 -85.497
WSB/EiJ m 217.3678 102.4843 420.3694 14.3661


  LEAST SQUARES MEANS (MODEL-ADJUSTED), SEXES COMBINED
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ both 711.2585 65.8217 841.6387 580.8783
A/J both 337.9708 67.1679 471.0175 204.9241
C57BL/6J both 364.2704 77.4708 517.7252 210.8156
CAST/EiJ both 145.5795 85.5255 314.989 -23.83
NOD/ShiLtJ both 169.638 93.086 354.0234 -14.7474
NZO/HlLtJ both 389.9194 79.561 547.5144 232.3244
PWK/PhJ both 260.8393 79.384 418.0837 103.5949
WSB/EiJ both 173.5933 74.6976 321.5549 25.6317




  GWAS USING LINEAR MIXED MODELS