Project measure / variable:   GMC20   IgG2a_17

ID, description, units MPD:56908   IgG2a_17   immunoglobulin G2a (plasma IgG2a)   [µg/mL]  at age 16-17wks  
Data set, strains GMC20   inbred w/CC8   7 strains     sex: both     age: 16-17wks
Procedure immunoglobulin quantification
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  STRAIN COMPARISON PLOT
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GMC20 - immunoglobulin G2a (plasma IgG2a) at age 16-17wks



  MEASURE SUMMARY
Measure Summary FemaleMale
Number of strains tested6 strains7 strains
Mean of the strain means176   µg/mL 163   µg/mL
Median of the strain means156   µg/mL 77.5   µg/mL
SD of the strain means± 137 ± 214
Coefficient of variation (CV)0.780 1.31
Min–max range of strain means40.2   –   390   µg/mL 43.9   –   643   µg/mL
Mean sample size per strain3.3   mice 3.1   mice


  ANOVA, Q-Q NORMALITY ASSESSMENT
ANOVA summary      
FactorDFSum of squaresMean sum of squaresF valuep value (Pr>F)
sex 1 274.6491 274.6491 0.0078 0.93
strain 4 726133.5777 181533.3944 5.1869 0.003
sex:strain 4 478651.7823 119662.9456 3.4191 0.0214
Residuals 28 979962.594 34998.6641


Q-Q normality assessment based on residuals

  


  STRAIN MEANS (UNADJUSTED)
  
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Strain Sex Mean SD N mice SEM CV Min, Max Z score
129S1/SvImJ f 248.0 142.0   4 70.9 0.572 118.0, 440.0 0.52
129S1/SvImJ m 643.0 496.0   4 248.0 0.771 200.0, 1268.0 2.25
A/J f 233.0 161.0   4 80.3 0.688 132.0, 471.0 0.42
A/J m 120.0 56.7   4 28.3 0.472 62.8, 197.0 -0.2
C57BL/6J f 78.1 18.7   3 10.8 0.239 64.9, 99.5 -0.71
C57BL/6J m 77.2 34.4   3 19.9 0.446 37.6, 99.5 -0.4
NOD/ShiLtJ f 66.9 18.5   4 9.26 0.277 50.0, 89.9 -0.8
NOD/ShiLtJ m 77.5 8.89   4 4.44 0.115 65.7, 85.5 -0.4
NZO/HlLtJ f 390.0 171.0   4 85.6 0.439 156.0, 567.0 1.56
NZO/HlLtJ m 124.0 33.7   4 16.8 0.27 92.4, 155.0 -0.18
PWK/PhJ m 58.0 0.0   1   0.0 0.0 58.0, 58.0 -0.49
WSB/EiJ f 40.2 0.0   1   0.0 0.0 40.2, 40.2 -0.99
WSB/EiJ m 43.9 0.481   2   0.34 0.0109 43.6, 44.3 -0.56


  LEAST SQUARES MEANS (MODEL-ADJUSTED)
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ f 247.815 93.5396 439.4223 56.2077
129S1/SvImJ m 642.5625 93.5396 834.1698 450.9552
A/J f 233.2725 93.5396 424.8798 41.6652
A/J m 120.0825 93.5396 311.6898 -71.5248
C57BL/6J f 78.1067 108.0103 299.3557 -143.1424
C57BL/6J m 77.2233 108.0103 298.4724 -144.0257
NOD/ShiLtJ f 66.905 93.5396 258.5123 -124.7023
NOD/ShiLtJ m 77.47 93.5396 269.0773 -114.1373
NZO/HlLtJ f 390.365 93.5396 581.9723 198.7577
NZO/HlLtJ m 124.445 93.5396 316.0523 -67.1623


  LEAST SQUARES MEANS (MODEL-ADJUSTED), SEXES COMBINED
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ both 445.1888 66.1425 580.6756 309.7019
A/J both 176.6775 66.1425 312.1643 41.1907
C57BL/6J both 77.665 76.3748 234.1117 -78.7817
NOD/ShiLtJ both 72.1875 66.1425 207.6743 -63.2993
NZO/HlLtJ both 257.405 66.1425 392.8918 121.9182




  GWAS USING LINEAR MIXED MODELS