Project measure / variable:   Auwerx2   ogtt_ins_t30_HFD

ID, description, units MPD:114606   ogtt_ins_t30_HFD   insulin, glucose tolerance test (20% glucose oral gavage, overnight fast), after 30 min   [µg/L]  HFD  
Data set, strains Auwerx2   inbred w/CC8   8 strains     sex: both     age: 16wks
Procedure hormone quantification
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  STRAIN COMPARISON PLOT
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Auwerx2 - insulin, glucose tolerance test (20% glucose oral gavage, overnight fast), after 30 min HFD



  MEASURE SUMMARY
Measure Summary FemaleMale
Number of strains tested8 strains8 strains
Mean of the strain means1.1467   µg/L 1.5591   µg/L
Median of the strain means0.62647   µg/L 1.4714   µg/L
SD of the strain means± 1.1133 ± 1.0102
Coefficient of variation (CV)0.9709 0.6479
Min–max range of strain means0.26288   –   3.5873   µg/L 0.5378   –   3.7837   µg/L
Mean sample size per strain4.2   mice 4.1   mice


  ANOVA, Q-Q NORMALITY ASSESSMENT
ANOVA summary      
FactorDFSum of squaresMean sum of squaresF valuep value (Pr>F)
sex 1 1.8566 1.8566 2.5035 0.1198
strain 7 70.8527 10.1218 13.6482 < 0.0001
sex:strain 7 4.8852 0.6979 0.941 0.4837
Residuals 51 37.8228 0.7416


Q-Q normality assessment based on residuals

  


  STRAIN MEANS (UNADJUSTED)
  
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Strain Sex Mean SD N mice SEM CV Min, Max Z score
129S1/SvImJ f 0.4602 0.27071   5 0.12107 0.5882 0.2085, 0.8995 -0.62
129S1/SvImJ m 1.3842 0.55048   2   0.38925 0.3977 0.995, 1.7735 -0.17
A/J f 0.68312 0.57945   4 0.28972 0.8482 0.324, 1.548 -0.42
A/J m 0.5378 0.37487   5 0.16765 0.697 0.184, 1.0755 -1.01
C57BL/6J f 0.537 0.22816   3 0.13173 0.4249 0.392, 0.8 -0.55
C57BL/6J m 1.7764 0.72209   4 0.36104 0.4065 0.7775, 2.4735 0.22
CAST/EiJ f 0.26288 0.13405   4 0.06703 0.5099 0.157, 0.4475 -0.79
CAST/EiJ m 1.5587 0.17713   2   0.12525 0.1136 1.4335, 1.684 0.0
DBA/2J f 3.5873 2.339   5 1.046 0.652 0.5825, 7.031 2.19
DBA/2J m 3.7837 1.2749   5 0.57013 0.3369 2.282, 5.5075 2.2
NOD/ShiLtJ f 1.187 0.49144   6 0.20063 0.414 0.649, 2.0615 0.04
NOD/ShiLtJ m 1.1858 0.5356   5 0.23953 0.4517 0.709, 2.012 -0.37
PWK/PhJ f 1.886 0.61687   4 0.30844 0.3271 1.155, 2.496 0.66
PWK/PhJ m 1.6496 0.61923   5 0.27693 0.3754 0.7225, 2.371 0.09
WSB/EiJ f 0.56983 0.47915   3 0.27664 0.8409 0.188, 1.1075 -0.52
WSB/EiJ m 0.5969 0.08541   5 0.0382 0.1431 0.4845, 0.716 -0.95


  LEAST SQUARES MEANS (MODEL-ADJUSTED)
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ f 0.4602 0.3851296745 1.233380084 -0.312980084
129S1/SvImJ m 1.38425 0.608943483 2.6067550535 0.1617449465
A/J f 0.683125 0.4305880662 1.5475666134 -0.1813166134
A/J m 0.5378 0.3851296745 1.310980084 -0.235380084
C57BL/6J f 0.537 0.4972002718 1.5351711963 -0.4611711963
C57BL/6J m 1.776375 0.4305880662 2.6408166134 0.9119333866
CAST/EiJ f 0.262875 0.4305880662 1.1273166134 -0.6015666134
CAST/EiJ m 1.55875 0.608943483 2.7812550535 0.3362449465
DBA/2J f 3.5873 0.3851296745 4.360480084 2.814119916
DBA/2J m 3.7837 0.3851296745 4.556880084 3.010519916
NOD/ShiLtJ f 1.187 0.3515736838 1.8928136217 0.4811863783
NOD/ShiLtJ m 1.1858 0.3851296745 1.958980084 0.412619916
PWK/PhJ f 1.886 0.4305880662 2.7504416134 1.0215583866
PWK/PhJ m 1.6496 0.3851296745 2.422780084 0.876419916
WSB/EiJ f 0.5698333333 0.4972002718 1.5680045297 -0.428337863
WSB/EiJ m 0.5969 0.3851296745 1.370080084 -0.176280084


  LEAST SQUARES MEANS (MODEL-ADJUSTED), SEXES COMBINED
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ both 0.922225 0.3602558229 1.6454687432 0.1989812568
A/J both 0.6104625 0.2888472559 1.190347563 0.030577437
C57BL/6J both 1.1566875 0.3288670678 1.8169156879 0.4964593121
CAST/EiJ both 0.9108125 0.3729002039 1.6594408973 0.1621841027
DBA/2J both 3.6855 0.2723278045 4.2322208805 3.1387791195
NOD/ShiLtJ both 1.1864 0.2607340222 1.7098453914 0.6629546086
PWK/PhJ both 1.7678 0.2888472559 2.347685063 1.187914937
WSB/EiJ both 0.5833666667 0.3144570625 1.2146655617 -0.0479322284




  GWAS USING LINEAR MIXED MODELS