MPD: Data set: Auwerx2

Auwerx2: Genetic, metabolic, and molecular insights into the diverse outcomes of diet-induced obesity in Collaborative Cross founder strains of mice (2021)

Bachmann AM, Morel JD, El Alam G, Rodríguez-López S, Imamura de Lima T, Goeminne LJE, Benegiamo G, Loric S, Conti M, Sleiman MB, Auwerx J. Genetic background and sex control the outcome of high-fat diet feeding in mice. iScience. 2022 Jun 17;25(6):104468. doi: 10.1016/j.isci.2022.104468. Epub 2022 May 25. PubMed 35677645 FullText

Male and female mice from nine inbred (CC) strains were fed either a standard chow diet (CD) or a high-fat diet (HFD) from 8 to 21 weeks of age. The effect of diet and sex was investigated on various physiological and metabolic traits over the course of the study. This included body weight as well as weights of organs such as cecum, skeletal muscle, heart, heart atria, kidneys, liver, spleen, and fat pads. The effects of an HFD diet were also assessed on cardiovascular function, respiratory function, activity, food intake, blood and insulin levels, and body temperature upon exposure to cold. The effect on blood plasma circulating lipids, free fatty acids, urea and transaminases were also investigated.

Experimental groups in this study:
• Chow diet (CD) • High fat diet (HFD)

Procedures conducted:

• body weight
• monitoring system		Oxygen consumption, carbon dioxide production, respiratory exchange rate (RER); activity.
• glucose tolerance		Plasma glucose levels: 0, 15, 30, 45, 60, 90, 120, 150, and 180 min, post oral gavage following overnight fast.
• hormone quantification		Insulin levels following glucose post oral gavage: 0, 15, 30 min.
• intake monitoring		Food intake.
• treadmill endurance test		Distance traveled.
• organ weights		Cecum, muscles, heart, heart-atria, kidney, liver, spleen, fat pads.
• organ dimensions		Intestine length.
• metabolic panel		Enzymes, creatinine, iron, lactate, urea.
• lipid profile		Cholesterol, HDL, LDL, triglycerides, free fatty acids.

Auwerx2 downloads
• Download Auwerx2 project data set animal data, as uploaded
• Download Auwerx2 animal data matrix with factor-related expansion applied as necessary
• Download Auwerx2 strain means, SD, N, etc. one row per strain/sex/measure

Ontology terms mapped to Auwerx2 measures:

Investigators	Johan Auwerx Ecole polytechnique fédérale de Lausanne, Lausanne SWITZERLAND Alexis M Bachmann Ecole polytechnique fédérale de Lausanne, Lausanne SWITZERLAND Jean-David Morel Ecole polytechnique fédérale de Lausanne, Lausanne SWITZERLAND Xiaoxu Li Ecole polytechnique fédérale de Lausanne, Lausanne SWITZERLAND
Participants	El Alam G, Rodríguez-López S, Imamura de Lima T, Goeminne L, Benegiamo G, Bou Sleiman M
Contact	Johan Auwerx admin.auwerx@epfl.ch Lab web site
Funding Provided By	ERC AdG-787702, SNSF 31003A_179435, NRF 2017K1A1A2013124, the Marie Skłodowska-Curie Individual Fellowship (896042), and the Strategic Focal Area PHRT of the ETH Domain (#2018-422)
Project type	Phenotype strain survey data set
MPD identifiers	Auwerx2 MPD:1110
Data changelog	No updates/corrections. Initial release date: 06/2022.
Formatted citation	Click above to copy-paste the entire citation for this MPD web page.

Mice: inbred w/CC8 9 strains ♀♂ age 8-21 wks 2 experimental groups

Sort/ Pick	Data set	Procedure / Protocol	Treatment	Phenotype Measure / Variable (click to view)	Panel	No. of Strains	Sex	Age	Sample Size (Avg)
Auwerx2:3	Auwerx2	body weight	(none)	body weight at 8 wks of age [g] bw_8wks 114531 CD HFD	inbred w/CC8	9	both	8wks	N=4

Auwerx2:5	Auwerx2	body weight	(none)	body weight at 9 wks of age [g] bw_9wks 114533 CD HFD	inbred w/CC8	9	both	9wks	N=4

Auwerx2:7	Auwerx2	body weight	(none)	body weight at 10 wks of age [g] bw_10wks 114535 CD HFD	inbred w/CC8	9	both	10wks	N=4

Auwerx2:9	Auwerx2	body weight	(none)	body weight at 11 wks of age [g] bw_11wks 114537 CD HFD	inbred w/CC8	9	both	11wks	N=4

Auwerx2:11	Auwerx2	body weight	(none)	body weight at 12 wks of age [g] bw_12wks 114539 CD HFD	inbred w/CC8	9	both	12wks	N=4

Auwerx2:13	Auwerx2	body weight	(none)	body weight at 13 wks of age [g] bw_13wks 114541 CD HFD	inbred w/CC8	9	both	13wks	N=4

Auwerx2:15	Auwerx2	body weight	(none)	body weight at 14 wks of age [g] bw_14wks 114543 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:17	Auwerx2	body weight	(none)	body weight at 15 wks of age [g] bw_15wks 114545 CD HFD	inbred w/CC8	9	both	15wks	N=4

Auwerx2:19	Auwerx2	body weight	(none)	body weight at 16 wks of age [g] bw_16wks 114547 CD HFD	inbred w/CC8	9	both	16wks	N=4

Auwerx2:21	Auwerx2	body weight	(none)	body weight at 17 wks of age [g] bw_17wks 114549 CD HFD	inbred w/CC8	9	both	17wks	N=4

Auwerx2:23	Auwerx2	body weight	(none)	body weight at 18 wks of age [g] bw_18wks 114551 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:25	Auwerx2	body weight	(none)	body weight at 19 wks of age [g] bw_19wks 114553 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:27	Auwerx2	body weight	(none)	body weight at 20 wks of age [g] bw_20wks 114555 CD HFD	inbred w/CC8	9	both	20wks	N=4

Auwerx2:29	Auwerx2	body weight	(none)	body weight at 21 wks of age [g] bw_21wks 114557 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:31	Auwerx2	body weight	(none)	body weight measured by EchoMRI [g] bw_14wks_MRI 114559 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:33	Auwerx2	MRI	(none)	fat tissue mass [g] fat 114561 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:35	Auwerx2	MRI	(none)	lean tissue mass [g] ltm 114563 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:37	Auwerx2	MRI	(none)	percentage fat tissue mass [%] fat_pct 114565 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:39	Auwerx2	MRI	(none)	percentage lean tissue mass [%] ltm_pct 114567 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:41	Auwerx2	monitoring system	(none)	oxygen consumption, 24h test, day time average [mL/kg/min] vo2_day 114569 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:43	Auwerx2	monitoring system	(none)	oxygen consumption, 24h test, night time average [mL/kg/min] vo2_night 114571 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:45	Auwerx2	monitoring system	(none)	carbon dioxide production, 24h test, day time average [mL/kg/min] vco2_day 114573 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:47	Auwerx2	monitoring system	(none)	carbon dioxide production, 24h test, night time average [mL/kg/min] vco2_night 114575 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:49	Auwerx2	monitoring system	(none)	respiratory exchange rate (VCO2:VO2), 24h test, day time average [ratio] rer_day 114577 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:51	Auwerx2	monitoring system	(none)	respiratory exchange rate (VCO2:VO2), 24h test, night time average [ratio] rer_night 114579 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:53	Auwerx2	body weight	(none)	body weight loss following 16h fast, prior to oral glucose tolerance test (OGTT), 16 wks of age [g] bw_16wks_ogtt 114581 CD HFD	inbred w/CC8	9	both	16wks	N=4

Auwerx2:55	Auwerx2	glucose tolerance	(none)	glucose baseline, glucose tolerance test (pregavage, overnight fast) [mg/dL] ogtt_gluc_t0 114583 CD HFD	inbred w/CC8	9	both	16wks	N=4

Auwerx2:57	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 15 min [mg/dL] ogtt_gluc_t15 114585 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:59	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 30 min [mg/dL] ogtt_gluc_t30 114587 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:61	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 45 min [mg/dL] ogtt_gluc_t45 114589 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:63	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 60 min [mg/dL] ogtt_gluc_t60 114591 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:65	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 90 min [mg/dL] ogtt_gluc_t90 114593 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:67	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 120 min [mg/dL] ogtt_gluc_t120 114595 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:69	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 150 min [mg/dL] ogtt_gluc_t150 114597 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:71	Auwerx2	glucose tolerance	(none)	glucose, glucose tolerance test (20% glucose oral gavage, overnight fast), after 180 min [mg/dL] ogtt_gluc_t180 114599 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:73	Auwerx2	hormone quantification	(none)	insulin baseline, glucose tolerance test (pregavage, overnight fast) [µg/L] ogtt_ins_t0 114601 CD HFD	inbred w/CC8	9	both	16wks	N=4

Auwerx2:75	Auwerx2	hormone quantification	(none)	insulin, glucose tolerance test (20% glucose oral gavage, overnight fast), after 15 min [µg/L] ogtt_ins_t15 114603 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:77	Auwerx2	hormone quantification	(none)	insulin, glucose tolerance test (20% glucose oral gavage, overnight fast), after 30 min [µg/L] ogtt_ins_t30 114605 CD HFD	inbred w/CC8	8	both	16wks	N=4

Auwerx2:79	Auwerx2	body temperature	(none)	body temperature (rectal) in cold (4°C) room, 0h exposed to cold [°C] temp_t0 114607 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:81	Auwerx2	body temperature	(none)	body temperature (rectal) in cold (4°C) room, 1h exposed to cold [°C] temp_t1 114609 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:83	Auwerx2	body temperature	(none)	body temperature (rectal) in cold (4°C) room, 2h exposed to cold [°C] temp_t2 114611 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:85	Auwerx2	body temperature	(none)	body temperature (rectal) in cold (4°C) room, 3h exposed to cold [°C] temp_t3 114613 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:87	Auwerx2	body temperature	(none)	body temperature (rectal) in cold (4°C) room, 4h exposed to cold [°C] temp_t4 114615 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:89	Auwerx2	body temperature	(none)	body temperature (rectal) in cold (4°C) room, 5h exposed to cold [°C] temp_t5 114617 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:91	Auwerx2	body temperature	(none)	body temperature (rectal) in cold (4°C) room, 6h exposed to cold [°C] temp_t6 114619 CD HFD	inbred w/CC8	9	both	18wks	N=4

Auwerx2:93	Auwerx2	body weight	(none)	body weight, prior to basal activity test, at 19 wks of age [g] bw_19wks_basal 114621 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:95	Auwerx2	monitoring system	(none)	horizontal activity (beam breaks) during day, basal activity test, for 48h [n/h] horiz_act_day 114623 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:97	Auwerx2	monitoring system	(none)	vertical activity (beam breaks) during day, basal activity test, for 48h [n/h] vert_act_day 114625 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:99	Auwerx2	monitoring system	(none)	horizontal activity (beam breaks) at night, basal activity test, for 48h [n/h] horiz_act_night 114627 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:101	Auwerx2	monitoring system	(none)	vertical activity (beam breaks) at night, basal activity test, for 48h [n/h] vert_act_night 114629 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:103	Auwerx2	intake monitoring	(none)	daily food intake, basal activity test, averaged over 2 days [g] food_intake 114631 CD HFD	inbred w/CC8	9	both	14wks	N=4

Auwerx2:105	Auwerx2	monitoring system	(none)	basal oxygen consumption, prior to treadmill test [mL/kg/min] vo2_basal 114633 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:107	Auwerx2	monitoring system	(none)	basal carbon dioxide production, prior to treadmill test [mL/kg/min] vco2_basal 114635 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:109	Auwerx2	monitoring system	(none)	basal respiratory exchange ratio (RER), prior to treadmill test (VCO2:VO2), 15 min time period, last 2 min analyzed [ratio] rer_basal 114637 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:111	Auwerx2	body weight	(none)	body weight, treadmill test [g] bw_treadmill 114639 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:113	Auwerx2	monitoring system	(none)	maximum oxygen consumption, treadmill test [ratio] vo2_max_treadmill 114641 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:115	Auwerx2	monitoring system	(none)	maximum carbon dioxide production, treadmill test [mL/kg/min] vco2_max_treadmill 114643 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:117	Auwerx2	monitoring system	(none)	maximum respiratory exchange ratio (RER), treadmill test (VCO2:VO2) [ratio] rer_max_treadmill 114645 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:119	Auwerx2	treadmill endurance test	(none)	distance traveled, treadmill test [m] dist_treadmill 114647 CD HFD	inbred w/CC8	9	both	19wks	N=4

Auwerx2:121	Auwerx2	body weight	(none)	body weight at sacrifice [g] bw_21wks_sac 114649 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:123	Auwerx2	organ weights	(none)	cecum weight [g] cecum_wt 114651 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:125	Auwerx2	organ weights	(none)	gastrocnemius muscle weight [g] gastroc_wt 114653 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:127	Auwerx2	organ weights	(none)	heart weight [g] heart_wt 114655 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:129	Auwerx2	organ weights	(none)	heart atria weight [g] heart_atria_wt 114657 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:131	Auwerx2	organ weights	(none)	kidney weight (both) [g] kidney_wt 114659 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:133	Auwerx2	organ weights	(none)	liver weight [g] liver_wt 114661 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:135	Auwerx2	organ weights	(none)	spleen weight [g] spleen_wt 114663 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:137	Auwerx2	organ weights	(none)	gonadal fat pad weight [g] eWAT 114665 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:139	Auwerx2	organ weights	(none)	subcutaneous adipose tissue weight [g] subcut_adip_wt 114667 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:141	Auwerx2	organ dimensions	(none)	intestine length [cm] intestine_length 114669 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:143	Auwerx2	metabolic panel	(none)	plasma alanine transaminase amount (ALAT) [U/L] ALAT 114671 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:145	Auwerx2	metabolic panel	(none)	plasma aspartate transaminase amount (ASAT) [U/L] ASAT 114673 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:147	Auwerx2	metabolic panel	(none)	plasma creatinine amount [µmol/L] creatinine 114675 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:149	Auwerx2	metabolic panel	(none)	plasma iron amount [mmol/L] iron 114677 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:151	Auwerx2	metabolic panel	(none)	plasma lactate dehydrogenase amount (LDH) [U/L] LDH 114679 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:153	Auwerx2	metabolic panel	(none)	plasma lactate amount [mmol/L] lactate 114681 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:155	Auwerx2	metabolic panel	(none)	plasma urea nitrogen amount [mmol/L] urea 114683 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:157	Auwerx2	lipid profile	(none)	plasma cholesterol amount [mmol/L] chol 114685 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:159	Auwerx2	lipid profile	(none)	plasma HDL amount [mmol/L] hdl 114687 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:161	Auwerx2	lipid profile	(none)	plasma LDL amount [mmol/L] ldl 114689 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:163	Auwerx2	lipid profile	(none)	plasma triglyceride amount [mmol/L] triglyc 114691 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2:165	Auwerx2	lipid profile	(none)	plasma free fatty acids amount [mmol/L] fatty_acids 114693 CD HFD	inbred w/CC8	9	both	21wks	N=4

Auwerx2: Genetic, metabolic, and molecular insights into the diverse outcomes of diet-induced obesity in Collaborative Cross founder strains of mice (2021)

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