Auwerx2: Genetic, metabolic, and molecular insights into the diverse outcomes of diet-induced obesity in Collaborative Cross founder strains of mice (2021)

Bachmann AM, Morel JD, El Alam G, Rodríguez-López S, Imamura de Lima T, Goeminne LJE, Benegiamo G, Loric S, Conti M, Sleiman MB, Auwerx J. Genetic background and sex control the outcome of high-fat diet feeding in mice. iScience. 2022 Jun 17;25(6):104468. doi: 10.1016/j.isci.2022.104468. Epub 2022 May 25.   PubMed 35677645     FullText

Auwerx2 downloads
• Download Auwerx2 project data set     animal data, as uploaded
• Download Auwerx2 animal data matrix     with factor-related expansion applied as necessary
• Download Auwerx2 strain means, SD, N, etc.     one row per strain/sex/measure
Ontology terms mapped to Auwerx2 measures:
  MA:0000009   adipose tissue
  MA:0000059   blood
  MA:0000072   heart
  MA:0000073   heart atrium
  MA:0000141   spleen
  MA:0000327   respiratory system
  MA:0000328   intestine
  MA:0000334   cecum
  MA:0000358   liver
  MA:0000368   kidney
  MA:0000473   subcutaneous adipose tissue
  MA:0002306   gastrocnemius
  MA:0002501   plasma
  MA:0002547   gonadal fat pad
  MP:0000180   abnormal circulating cholesterol level
  MP:0000181   abnormal circulating LDL cholesterol level
  MP:0000184   abnormal circulating HDL cholesterol level
  MP:0000187   abnormal triglyceride level
  MP:0000203   abnormal circulating aspartate transaminase level
  MP:0001259   abnormal body weight
  MP:0001392   abnormal locomotor behavior
  MP:0001553   abnormal circulating free fatty acids level
  MP:0001560   abnormal circulating insulin level
  MP:0001573   abnormal circulating alanine transaminase level
  MP:0002332   abnormal exercise endurance
  MP:0002336   abnormal pulmonary gas exchange
  MP:0002707   abnormal kidney weight
  MP:0002943   abnormal lactate dehydrogenase level
  MP:0003959   abnormal lean body mass
  MP:0004152   abnormal circulating iron level
  MP:0004847   abnormal liver weight
  MP:0004857   abnormal heart weight
  MP:0004951   abnormal spleen weight
  MP:0005265   abnormal blood urea nitrogen level
  MP:0005288   abnormal oxygen consumption
  MP:0005291   abnormal glucose tolerance
  MP:0005335   abnormal gonadal fat pad morphology
  MP:0005449   abnormal food intake
  MP:0005452   abnormal adipose tissue amount
  MP:0005457   abnormal percent body fat/body weight
  MP:0005535   abnormal body temperature
  MP:0008962   abnormal carbon dioxide production
  MP:0010060   abnormal creatine level
  MP:0012687   abnormal heart atrium weight
  MP:0013188   abnormal gastrocnemius weight
  MP:0013403   abnormal circulating lactate level
  MP:0020997   abnormal subcutaneous adipose tissue amount
  MP:0021000   abnormal cecum weight
  VT:0000180   blood cholesterol amount
  VT:0000181   blood LDL cholesterol amount
  VT:0000184   blood HDL cholesterol amount
  VT:0000188   blood glucose amount
  VT:0000203   blood aspartate transaminase amount
  VT:0001259   body mass
  VT:0001392   locomotor behavior trait
  VT:0001431   eating behavior trait
  VT:0001553   blood free fatty acid amount
  VT:0001560   blood insulin amount
  VT:0001573   blood alanine transaminase amount
  VT:0001777   body temperature regulation trait
  VT:0002121   metabolism trait
  VT:0002332   exercise endurance trait
  VT:0002336   pulmonary gas exchange trait
  VT:0002338   pulmonary ventilation trait
  VT:0002644   blood triglyceride amount
  VT:0002707   kidney mass
  VT:0003402   liver mass
  VT:0004152   blood iron amount
  VT:0004951   spleen mass
  VT:0005265   blood urea nitrogen amount
  VT:0005328   blood creatinine amount
  VT:0005452   adipose amount
  VT:0007028   heart mass
  VT:0010131   cecum mass
  VT:0010195   intestine length
  VT:0010423   gonadal fat pad mass
  VT:0010471   exploratory behavior trait
  VT:0010477   blood lactate dehydrogenase amount
  VT:0010482   body fat mass
  VT:0010483   body lean mass
  VT:0010616   blood lactate amount
  VT:0010785   blood carbon dioxide amount
  VT:0015088   glucose metabolism trait
  VT:1000472   subcutaneous adipose mass

Investigators Johan Auwerx       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Alexis M Bachmann       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Jean-David Morel       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Xiaoxu Li       Ecole polytechnique fédérale de Lausanne,  Lausanne SWITZERLAND
Participants El Alam G, Rodríguez-López S, Imamura de Lima T, Goeminne L, Benegiamo G, Bou Sleiman M
ContactJohan Auwerx     Lab web site
Funding Provided ByERC AdG-787702, SNSF 31003A_179435, NRF 2017K1A1A2013124, the Marie Skłodowska-Curie Individual Fellowship (896042), and the Strategic Focal Area PHRT of the ETH Domain (#2018-422)
Project type Phenotype strain survey data set
MPD identifiersAuwerx2     MPD:1110
Data changelog No updates/corrections.       Initial release date: 06/2022.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
Male and female mice from nine inbred (CC) strains were fed either a standard chow diet (CD) or a high-fat diet (HFD) from 8 to 21 weeks of age. The effect of diet and sex was investigated on various physiological and metabolic traits over the course of the study. This included body weight as well as weights of organs such as cecum, skeletal muscle, heart, heart atria, kidneys, liver, spleen, and fat pads. The effects of an HFD diet were also assessed on cardiovascular function, respiratory function, activity, food intake, blood and insulin levels, and body temperature upon exposure to cold. The effect on blood plasma circulating lipids, free fatty acids, urea and transaminases were also investigated.

Experimental groups in this study:
• Chow diet (CD)     • High fat diet (HFD)    

Procedures conducted:
• body weight 
• monitoring system  Oxygen consumption, carbon dioxide production, respiratory exchange rate (RER); activity.
• glucose tolerance  Plasma glucose levels: 0, 15, 30, 45, 60, 90, 120, 150, and 180 min, post oral gavage following overnight fast.
• hormone quantification  Insulin levels following glucose post oral gavage: 0, 15, 30 min.
• intake monitoring  Food intake.
• treadmill endurance test  Distance traveled.
• organ weights  Cecum, muscles, heart, heart-atria, kidney, liver, spleen, fat pads.
• organ dimensions  Intestine length.
• metabolic panel  Enzymes, creatinine, iron, lactate, urea.
• lipid profile  Cholesterol, HDL, LDL, triglycerides, free fatty acids.

Mice: inbred w/CC8   9 strains   ♀♂   age 8-21 wks   2 experimental groups