Project measure / variable:   Auwerx2   ogtt_ins_t15_HFD

ID, description, units MPD:114604   ogtt_ins_t15_HFD   insulin, glucose tolerance test (20% glucose oral gavage, overnight fast), after 15 min   [µg/L]  HFD  
Data set, strains Auwerx2   inbred w/CC8   8 strains     sex: both     age: 16wks
Procedure hormone quantification
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  STRAIN COMPARISON PLOT
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Auwerx2 - insulin, glucose tolerance test (20% glucose oral gavage, overnight fast), after 15 min HFD



  MEASURE SUMMARY
Measure Summary FemaleMale
Number of strains tested8 strains8 strains
Mean of the strain means1.4201   µg/L 2.4472   µg/L
Median of the strain means0.91522   µg/L 2.1635   µg/L
SD of the strain means± 1.1052 ± 1.7146
Coefficient of variation (CV)0.7783 0.7006
Min–max range of strain means0.548   –   3.3324   µg/L 0.7005   –   5.3604   µg/L
Mean sample size per strain4.4   mice 4.0   mice


  ANOVA, Q-Q NORMALITY ASSESSMENT
ANOVA summary      
FactorDFSum of squaresMean sum of squaresF valuep value (Pr>F)
sex 1 20.1714 20.1714 13.8986 0.0005
strain 7 114.1561 16.308 11.2366 < 0.0001
sex:strain 7 14.3796 2.0542 1.4154 0.2197
Residuals 51 74.0179 1.4513


Q-Q normality assessment based on residuals

  


  STRAIN MEANS (UNADJUSTED)
  
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Strain Sex Mean SD N mice SEM CV Min, Max Z score
129S1/SvImJ f 0.5974 0.24584   5 0.10994 0.4115 0.282, 0.9025 -0.74
129S1/SvImJ m 0.9635 0.27931   2   0.1975 0.2899 0.766, 1.161 -0.87
A/J f 0.548 0.27736   4 0.13868 0.5061 0.379, 0.9625 -0.79
A/J m 0.7005 0.36379   5 0.16269 0.5193 0.3635, 1.2475 -1.02
C57BL/6J f 0.616 0.12292   3 0.07097 0.1995 0.497, 0.7425 -0.73
C57BL/6J m 2.6938 1.5233   4 0.76163 0.5655 0.753, 4.2485 0.14
CAST/EiJ f 1.0036 0.3586   5 0.16037 0.3573 0.5255, 1.4475 -0.38
CAST/EiJ m 3.0405 2.32   2   1.6405 0.763 1.4, 4.681 0.35
DBA/2J f 2.9289 1.5724   5 0.70321 0.5369 0.8215, 5.1565 1.37
DBA/2J m 5.3604 2.6658   5 1.1922 0.4973 1.9485, 9.233 1.7
NOD/ShiLtJ f 1.5077 0.62583   6 0.25549 0.4151 0.785, 2.59 0.08
NOD/ShiLtJ m 1.6331 0.79399   5 0.35508 0.4862 0.904, 2.524 -0.47
PWK/PhJ f 3.3324 0.84358   4 0.42179 0.2531 2.212, 4.0245 1.73
PWK/PhJ m 4.2841 1.9173   5 0.85744 0.4475 2.509, 7.2685 1.07
WSB/EiJ f 0.82683 0.35885   3 0.20718 0.434 0.535, 1.2275 -0.54
WSB/EiJ m 0.9015 0.22924   4 0.11462 0.2543 0.5875, 1.1355 -0.9


  LEAST SQUARES MEANS (MODEL-ADJUSTED)
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ f 0.5974 0.5387638217 1.6790135045 -0.4842135045
129S1/SvImJ m 0.9635 0.8518603988 2.6736811112 -0.7466811112
A/J f 0.548 0.6023562646 1.7572806608 -0.6612806608
A/J m 0.7005 0.5387638217 1.7821135045 -0.3811135045
C57BL/6J f 0.616 0.695541103 2.01235703 -0.78035703
C57BL/6J m 2.69375 0.6023562646 3.9030306608 1.4844693392
CAST/EiJ f 1.0036 0.5387638217 2.0852135045 -0.0780135045
CAST/EiJ m 3.0405 0.8518603988 4.7506811112 1.3303188888
DBA/2J f 2.9289 0.5387638217 4.0105135045 1.8472864955
DBA/2J m 5.3604 0.5387638217 6.4420135045 4.2787864955
NOD/ShiLtJ f 1.50775 0.4918218305 2.4951235249 0.5203764751
NOD/ShiLtJ m 1.6331 0.5387638217 2.7147135045 0.5514864955
PWK/PhJ f 3.332375 0.6023562646 4.5416556608 2.1230943392
PWK/PhJ m 4.2841 0.5387638217 5.3657135045 3.2024864955
WSB/EiJ f 0.8268333333 0.695541103 2.2231903634 -0.5695236967
WSB/EiJ m 0.9015 0.6023562646 2.1107806608 -0.3077806608


  LEAST SQUARES MEANS (MODEL-ADJUSTED), SEXES COMBINED
Strain Sex Mean SEM UpperCL LowerCL
129S1/SvImJ both 0.78045 0.5039674083 1.7922067897 -0.2313067897
A/J both 0.62425 0.4040728663 1.4354601284 -0.1869601284
C57BL/6J both 1.654875 0.4600571963 2.5784783607 0.7312716393
CAST/EiJ both 2.02205 0.5039674083 3.0338067897 1.0102932103
DBA/2J both 4.14465 0.3809635518 4.9094662437 3.3798337563
NOD/ShiLtJ both 1.570425 0.3647448316 2.3026808042 0.8381691958
PWK/PhJ both 3.8082375 0.4040728663 4.6194476284 2.9970273716
WSB/EiJ both 0.8641666667 0.4600571963 1.7877700274 -0.0594366941




  GWAS USING LINEAR MIXED MODELS