Project protocol — Contents
Workflow and sampling
Reagents, supplies, and solutions
Procedure for evaluating vaginal patency
Test Procedure performed Equipment/ Reagent Data collected 1 Female mice are examined daily - - 2 Each mouse is adequately restrained for examination forceps age at vaginal opening 3 Disinfect forcep with ethanol after each use ethanol -
- 95% ethanol solution
Procedure for evaluating age at vaginal opening
a. Female mice are examined daily starting at 15-day of age until vaginal patency is observed (see Figure 1 below).
b. Forceps are disinfected using 95% ethanol solution prior to vaginal examination.
c. With proper hand restraint, each mouse is positioned ventro-dorsally, and vaginal cavity is examined gently with clean forceps.
d. For additional information go to The Aging Center at The Jackson Laboratory.
Figure 1. Schematic illustration of vaginal patency in the female mouse.
Vaginal opening or patency is one example of postnatal tissue remodeling as part of normal development and reproductive maturation. This is similar to those leading to the opening of the eyes, and the ears shortly after birth in mice (see Rodriquez et. al., 1997).
Rodriguez I, Araki K, Khatib K, Martinou JC, Vassalli P. Mouse vaginal opening is an apoptosis-dependent process which can be prevented by the overexpression of Bcl2. Dev Biol. 1997 Apr 1;184(1):115-21.