Wittenburg2: Cholesterol gallstone formation in reciprocal backcrosses between PERA/EiJ and I/LnJ (2003)

References   (chronological)

1.   Wittenburg H, Lyons MA, Li R, Churchill GA, Carey MC, Paigen B. FXR and ABCG5/ABCG8 as determinants of cholesterol gallstone formation from quantitative trait locus mapping in mice. Gastroenterology. 2003 Sep;125(3):868-81.   PubMed 12949731  

2.   Li R, Lyons MA, Wittenburg H, Paigen B, Churchill GA. Combining data from multiple inbred line crosses improves the power and resolution of quantitative trait loci mapping. Genetics. 2005 Mar;169(3):1699-709. Epub 2005 Jan 16.   PubMed 15654110     FullText

3.   Wittenburg H, Lyons MA, Li R, Kurtz U, Wang X, Mössner J, Churchill GA, Carey MC, Paigen B. QTL mapping for genetic determinants of lipoprotein cholesterol levels in combined crosses of inbred mouse strains. J Lipid Res. 2006 Aug;47(8):1780-90. Epub 2006 May 9.   PubMed 16685081  


     
Wittenburg2 downloads
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Use the QTL Data Downloads button near the bottom of this page.
Participants H Wittenberg
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Lyons MA, Li R, Carey MC, Paigen B
ContactGary A Churchill     gary.churchill@jax.org
AffiliationChurchill Group QTL Archive
AcknowledgementsFunding provided by NIH DK51568, DK36588, DK52911, DK34854, CA34196, DK73687, GM070683, HL55001; Deutsche Forschungsgemeinschaft, American Physiological Society, and the American Liver Foundation, the National Health and Medical Research Council of Australia, the Gastroenterological Society of Australia.
Project type QTL Archive data set
MPD identifiersWittenburg2     MPD:138
Data changelog No updates/corrections.       Initial release date: 01/2008.
An intercross of PERA/EiJ and I/LnJ was generated to identify cholesterol gallstone susceptibility loci. F2 progeny of both sexes were administered a high-fat (15%) diet for 8 wks and examined for gallstone formation and related phenotypes. Gallstones were characterized qualitatively and quantitatively, and lipid profiles determined for plasma samples. Mice were fasted prior to sample collection. For detailed protocols see Wittenburg et al 2003.


QTL data set parameters:
Progenitor strains I/LnJ     PERA/EiJ    
Cross typeF2 intercross
Population size 164 ♀     141 ♂


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Select table page: First Prev Next Last
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Wittenburg2 13801 body weight (none) body weight, 6-8wks of age   [g]  bw_start QTL population both 6-8wks N=153
Wittenburg2 13802 body weight high-fat diet body weight change (bw end minus bw start), 14-16wks of age   [g]  bw_change QTL population both 14-16wks N=153
Wittenburg2 13803 organ weights high-fat diet liver weight   [g]  liver_wt QTL population both 14-16wks N=153
Wittenburg2 13804 organ weights high-fat diet percent of total body weight that is liver weight   [%]  liver_wt_pct QTL population both 14-16wks N=153
Wittenburg2 13805 organ dimensions high-fat diet gallbladder, volume   [µL]  gallbladder_vol QTL population both 14-16wks N=153
Wittenburg2 13806 histopathology high-fat diet gallbladder, mucin (score 0-5)   [score]  gallbladder_mucin QTL population both 14-16wks N=153
Wittenburg2 13807 histopathology high-fat diet gallstones, presence (score 0=absent, 1=present)   [score]  gallstone_presence QTL population both 14-16wks N=153
Wittenburg2 13808 histopathology high-fat diet gallstones, screening (score 0=absent, 1=present, 2=solid gallstones)   [score]  gallstone_screen QTL population both 14-16wks N=153
Wittenburg2 13809 histopathology high-fat diet number of solid gallstones   [n]  gallstone_count QTL population both 14-16wks N=153
Wittenburg2 13810 histopathology high-fat diet weight of solid gallstones   [mg]  gallstone_wt QTL population both 14-16wks N=153
Wittenburg2 13811 histopathology high-fat diet weight of solid gallstones, log transformed   [log(mg)]  gallstone_wt_log QTL population both 14-16wks N=153
Wittenburg2 13812 histopathology high-fat diet weight of solid gallstones ([mg] + 1), log10 transformed   [log(mg)]  gallstone_wt_log10 QTL population both 14-16wks N=153
Wittenburg2 13813 histopathology high-fat diet weight of solid gallstones, square root   [sqrt(mg)]  gallstone_wt_sqr QTL population both 14-16wks N=153
Wittenburg2 13814 histopathology high-fat diet gallstones, presence of sandy stones (score 0=absent, 1=present)   [score]  gallstone_sandy QTL population both 14-16wks N=153
Wittenburg2 13815 histopathology high-fat diet cholesterol monohydrate crystals (score 0-5)   [score]  gallstone_crystals QTL population both 14-16wks N=153
Wittenburg2 13816 histopathology high-fat diet gallstone severity (score 0-3)   [score]  gallstone_score QTL population both 14-16wks N=153
Wittenburg2 13817 lipid profile high-fat diet total cholesterol (plasma)   [mg/dL]  chol QTL population both 14-16wks N=153
Wittenburg2 13818 lipid profile high-fat diet HDL (plasma)   [mg/dL]  HDL QTL population both 14-16wks N=153
Wittenburg2 13819 lipid profile high-fat diet HDL (plasma), log transformed   [log(mg/dL)]  HDL_log QTL population both 14-16wks N=153
Wittenburg2 13820 lipid profile high-fat diet nonHDL (plasma)   [mg/dL]  nonHDL QTL population both 14-16wks N=153
To see more rows use the paging control at top.

Associated genomic features
MGI-curated genes / genomic features that have been associated with this data set:
Hdlq10   Hdlq33   Hdlq34   Hdlq35   Hdlq36   Hdlq37   Hdlq38   Hdlq39   Lith7   Lith8   Lith9   Nhdlq10   Nhdlq8   Nhdlq9  


Project / data set:   Wittenburg2     a.k.a. Wittenburg_2003
Excel file:   Data_Description_PERAxI_Wittenburg2003.xlsx
CSV file:   Wittenburg2003_PERAxI_B37_Data.csv
Readme / text file:   readme_Wittenburg2003.txt