Wittenburg1: Cholesterol gallstones, gallbladder mucin accumulation, and related gallstone phenotypes in reciprocal backcrosses between AKR/J and SWR/J (2002)

Wittenburg H, Lammert F, Wang DQ, Churchill GA, Li R, Bouchard G, Carey MC, Paigen B. Interacting QTLs for cholesterol gallstones and gallbladder mucin in AKR and SWR strains of mice. Physiol Genomics. 2002 Feb 11;8(1):67-77.   PubMed 11842132  


     
Wittenburg1 downloads
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Use the QTL Data Downloads button near the bottom of this page.
Participants H Wittenberg
Gary A Churchill       The Jackson Laboratory Bar Harbor, ME
With: Lammert F, Wang DQ, Li R, Bouchard G, Carey MC, Paigen B
ContactGary A Churchill     gary.churchill@jax.org
AffiliationChurchill Group QTL Archive
AcknowledgementsGrant: This work was supported in part by National Institute of Diabetes and Digestive and Kidney Diseases Grants DK-51568 (to B. Paigen), DK-36588, DK-52911, and DK-34854 (to M. C. Carey), DK-54012 (to D. Q.-H. Wang), and by a grant from the Ellison Medical Foundation (to D. Q.-H. Wang). F. Lammert (La 997/3-1) and H. Wittenburg (WI 1905/1-1) were supported by the Deutsche Forschungsgemeinschaft.
Project type QTL Archive data set
MPD identifiersWittenburg1     MPD:126
Data changelog No updates/corrections.       Initial release date: 01/2008.
A reciprocal backcross of AKR/J and SWR/J was generated to identify QTLs for cholesterol gallstones, gallbladder mucin accumulation, liver and body weight. At 8 wks of age, backcross progeny of both sexes were administered a high-fat (15%) diet for 12 wks and examined for gallstone formation and related phenotypes. Mice were fasted prior to sample collection. For detailed protocols see Wittenburg et al 2002.


QTL data set parameters:
Progenitor strains AKR/J     SWR/J    
Cross direction notation(SWR x AKR) x AKR; AKR x (SWR x AKR)
Cross typeReciprocal backcross
Population size 41 ♀     220 ♂
Age18-20wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
Wittenburg1 12601 body weight high-fat diet body weight, log transformed   [log(g)]  bw_log QTL population both 18-20wks N=131
Wittenburg1 12602 organ weights high-fat diet liver weight   [g]  liver_wt QTL population both 18-20wks N=131
Wittenburg1 12603 organ weights high-fat diet liver-to-bw ratio   [ratio]  liver_bw_ratio QTL population both 18-20wks N=131
Wittenburg1 12604 organ dimensions high-fat diet gallbladder, volume   [µL]  gallbladder_vol QTL population both 18-20wks N=131
Wittenburg1 12605 histopathology high-fat diet gallbladder, mucin (score 0-4), log transformed   [score]  gallbladder_mucin_log QTL population both 18-20wks N=131
Wittenburg1 12606 histopathology high-fat diet gallstones, presence (score 0=absent, 1=present)   [score]  gallstone_presence QTL population both 18-20wks N=131
Wittenburg1 12607 histopathology high-fat diet gallstones, number   [n]  gallstone_count QTL population both 18-20wks N=131
Wittenburg1 12608 histopathology high-fat diet weight of solid gallstones   [mg]  gallstone_wt QTL population both 18-20wks N=131
Wittenburg1 12609 histopathology high-fat diet gallstone severity (score 0=absent, 1=crystals, 2=stones)   [score]  gallstone_score QTL population both 18-20wks N=131
Wittenburg1 12610 histopathology high-fat diet gallstones volume  gallstone_vol QTL population both 18-20wks N=131

Associated genomic features
MGI-curated genes / genomic features that have been associated with this data set:
Lith1   Lith2   Lith3   Lith4   Lith5  


Project / data set:   Wittenburg1     a.k.a. Wittenburg_2002
Excel file:   Data_Description_SWRxAKR_BC_Wittenburg2002.xlsx
CSV file:   Wittenburg2002_SWRxAKR_BC_B37_Data.csv
Readme / text file:   readme_Wittenburg2002.txt