Acclimation & testing periods
Ages JAX® mice were housed in JAX production rooms until 9-12 weeks of age, thereafter, the mice were shipped to NEOCOM and immediately uppon arrival were euthanized for the study. Feed All strains maintained on a sterilized 6% fat diet (NIH316 formula, Purina item #5K52) from age of weaning. Water Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA). Housing Thoren cages; Thoren Maxi Miser #3 cages used for some strains as either part of a PIV (pressurized individually ventilated) system or placed on conventional racking. Bedding Steam autoclaved white pine shavings. Humidity ~50% Temperature 18-24 °C Photoperiod 14 hrs on (generally covering the hrs of 4:30 AM to 6:30 PM) 10 hrs off. Health status The Northeastern Ohio Universities Colleges of Medicine and Pharmacy (NEOUCOM) Institutional Animal Care and Use Committee approved all protocols used in this study.