Tomasini1 animal documentation

A survey of plasma fibronectin concentration in 25 inbred strains of mice   (2007)

Tomasini-Johansson BR, Mosher DF
With: Peters LL, Svenson KL

See also: Tomasini1 protocol


Tomasini1_Animal

Acclimation and testing periods

Vendor
Mice from the The Jackson Laboratory JAX® (Bar Harbor, ME) production facility were obtained and bled at TJL, and the frozen plasma were shipped and assayed at UWM
Ages
9 wks of age
Feed
Most strains maintained on LabDiet 5K52 (6% fat) during the acclimation period
Water
Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA).
Housing
Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system.
Bedding
Steam autoclaved white pine shavings.
Photoperiod
12 hours on (6 am to 6 pm), 12 hours off
Relative humidity ~50%
Temperature
24-25 °C
Health status
No pathogens detected in animal room. Mice appeared healthy during acclimation period. The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols. (Sentinel animals are tested). Detailed health status reports for each animal room represented from this project are on file and available on request. See JAX Mice Quality Control Programs or the JAX Mice Catalog for the list of agents monitored.