Svenson4 animal documentation

Bone mineral density and body composition in C57BL/6J-Chr#PWD/Ph/ForeJ mouse chromosome substitution strains   (2006)

Svenson KL, Forejt J, Donahue L, Paigen B
With: Marion M

See also: Svenson4 protocol


Svenson4 Animal Documentation

Acclimation & testing periods

Vendor
Mice were born, raised, and maintained at The Jackson Laboratory (JAX®, Bar Harbor, ME) research facility.
Acclimation Mice were acclimated at the HLB facility at 6-16-wks of age
Ages 16 wks of age at the time of testing
Feed LabDiet 5K52 (6% fat) during the acclimation period
Water Autoclaved and adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/ml) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA) ad-libitum
Housing Thoren cages; Thoren Maxi Miser #3 cages with a PIV (pressurized individually ventilated) system
Bedding Steam autoclaved white pine shavings from Crobb Box Co. of Ellsworth, ME
Photoperiod 12/12 Light/Dark; beginning at 6 AM
Temperature 18-24°C
Relative humidity ~50%
Health status Sentinel mice were periodically examined throughout the study and were free of microbiological infections; for additional information