Shorter7 project protocol

Male reproduction in 28 F1 hybrids of Collaborative Cross strains of mice (RIX): Sperm motility (CASA)   (2020)

Shorter JR, O'Brien DA, Pardo-Manuel de Villena F
With: Pan W, Odet F, Aylor DL, Kao C-Y, Fu C-P, Greenstein S, Bell TA, Stevans AM, Patel S, Cates SE, Shaw GD, MIller DR, McMillian L




Project protocol - Contents

Workflow and sampling

Step Description Equipment Data collected
1 Mice euthanized and weighed (see Shorter5) - -
2 Reproductive organs harvested and weighed (see Shorter5) - -
3 Sperm harvested from right cauda epididymis and counted (see Shorter5) - -
4 Sperm harvested from left cauda epididymis for in vitro capacitation study on sperm motility Microscope (Computer Assisted Sperm Analysis (CASA)) Motility analysis
5 Sperm from left cauda epididymis prepared to assess morphology (see Shorter5) - -

Procedures

General Information for All Procedures: See Shorter5 for reproductive organ weights, sperm count, motility and morphology.


References

Protocol References

Goodson SG, Zhang Z, Tsuruta JK, Wang W, O'Brien DA. Classification of mouse sperm motility patterns using an automated multiclass support vector machines model. Biol Reprod. 2011 Jun;84(6):1207-15. doi: 10.1095/biolreprod.110.088989. Epub 2011 Feb 23.   PubMed 21349820     FullText