Project
protocol
-
Contents
Workflow
and
sampling
Step |
Procedure |
Equipment |
Data
collected |
1 |
Administer diets |
- |
- |
2 |
Weigh and euthanize mice
|
Scales |
Body weight |
3 |
Harvest heart and weigh |
Scales |
Heart weight |
4 |
Harvest femur and measure |
Digital calipers |
Femur length |
Procedure: Diet administration
- Two cohorts of DO mice were used: Generation 10 (G10) and G11. G10 mice were administered the control diet upon arrival at the test facility at 4 wks of age and maintained on that diet until 12-15 wks of age when they were euthanized. G11 mice were split into two groups and administered the control diet at 4 wks of age for 2 wks and then maintained on their respective experimental diets until 24-25 wks of age (18-19 wks on the experimental diet) when they were euthanized.
- The high-fat, cholic acid (HFC) diet contained 20% fat, 1.25% cholesterol, and 0.5% cholic acid to induce atherosclerotic lesions.
- The low-fat, high-protein (LFHP) diet contained 5% fat and 20.3% protein. This diet is not atherogenic.
Procedure: Heart weight and femur length
- Mice are weighed then euthanized by CO2 asphyxiation followed by cervical dislocation.
- The heart is dissected from the chest cavity; atria and all adherent tissue are carefully excised prior to weighing the ventricles.
- Right hind legs are removed and fixed in 10% formalin; afterward, the femur is dissected and measured two independent times to 0.01 mm using digital calipers.
Data
collected
by
investigator
- Body weight
- Heart weight
- Heart weight adjusted to body weight
- Femur length
- Heart weight adjusted to femur length
|