Seburn1 animal documentation

Comprehensive metabolic survey of 16 inbred strains of mice   (2001)

Seburn KL

See also: Seburn1 protocol


Vendor: Mice obtained from The Jackson Laboratory.

Health status report: The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols.

See JAX®Mice Quality Control Programs or the JAX®Mice Catalog for the list of agents monitored. Detailed health status reports for each animal room represented from this project are on file. Contact us for specific reports.

Shipment age: Mice shipped 6-8 weeks of age to Dr. Seburn's facility at The Jackson Laboratory (Seburn1). Immediately following metabolic characterization, mice were shipped to Dr. Hampton's laboratory for ECG tests (Hampton1).

Shipment dates: Mice shipped from JAX to Dr. Seburn's laboratory 7-Jul-2000 to 2-Apr-2001.

Environment before shipping

Feed: Strains maintained on a 6% fat diet (NIH 31M).

Water: Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/mL) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA).

Housing: Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system.

Bedding: Steam autoclaved white pine shavings.

Photoperiod: 14 hours on (generally covering the hours of 0430 h to 1830 h) 10 hours off.

Temperature: 18 - 24°C

Relative humidity: ~50%.

Acclimation Period

Duration: 7-14 days (in non-SPF research facility room at The Jackson Laboratory).

Feed: Sterilized NIH 31M

Water: Adjusted with hydrochloric acid to pH 3.0; supplemented with vitamin K.

Housing: Thoren Duplex cages (no more than 10 mice / 51.7 sq. in. floor space).

Bedding: same as vendor (above).

Photoperiod: 14 hours on (on 0700 h to 2100 h) 10 hours off.

Temperature: 24 - 25 °C

Relative humidity: 47-49%

Health status report: Mice appeared healthy during acclimation period.

Testing Period

See protocols for Seburn1 and Hampton1.