Vendor: Mice obtained from The Jackson Laboratory.
Health status report: The Jackson Laboratory Diagnostics Laboratory routinely monitors and documents animal health status using statistically valid sampling procedures and standard protocols.
See JAX®Mice Quality Control Programs
or the JAX®Mice Catalog for the list of agents monitored.
status reports for each animal room represented from this
project are on file. Contact us for specific reports.
Shipment dates: Mice shipped from JAX to Dr. Seburn's laboratory 7-Jul-2000 to 2-Apr-2001.
Environment before shippingFeed: Strains maintained on a 6% fat diet (NIH 31M).
Water: Adjusted with hydrochloric acid to pH 2.8-3.1 and supplemented with menadione sodium bisulfite (final concentration 0.4 mg/mL) to compensate for potential decrease in vitamin K activity upon autoclaving; water supplied in pinhole type bottles (Thoren Caging Systems, Inc. Hazelton, PA).
Housing: Thoren cages; Thoren Maxi Miser #3 cages used for some strains as part of a PIV (pressurized individually ventilated) system.
Bedding: Steam autoclaved white pine shavings.
Photoperiod: 14 hours on (generally covering the hours of 0430 h to 1830 h) 10 hours off.
Temperature: 18 - 24°C
Relative humidity: ~50%.
Acclimation PeriodDuration: 7-14 days (in non-SPF research facility room at The Jackson Laboratory).
Feed: Sterilized NIH 31M
Water: Adjusted with hydrochloric acid to pH 3.0; supplemented with vitamin K.
Housing: Thoren Duplex cages (no more than 10 mice / 51.7 sq. in. floor space).
Bedding: same as vendor (above).
Photoperiod: 14 hours on (on 0700 h to 2100 h) 10 hours off.
Temperature: 24 - 25 °C
Relative humidity: 47-49%
Health status report: Mice appeared healthy during acclimation period.
Testing PeriodSee protocols for Seburn1 and Hampton1.