MPD: RIKEN-EAD2: animal documentation

RIKEN-EAD2 animal documentation

Blood chemistry, hematology, and body size for 33 RBRC wild-derived mouse strains (2008)

RIKEN BioResource Center / Experimental Animal Division
With: Yoshiki, A

RIKEN-EAD2_ Animal Documentation

Mouse Husbandry

Supplier RIKEN BioResource Center / Experimental Animal Division

Ages Mice were housed in RIKEN BRC barrier facility until 8 weeks old when sample collection took place.

Diet All strains maintained on a Gamma-irradiated commercial diet (CLEA Rodent Diet CE-2, Japan, Inc., Tokyo, Japan) CLEA

Water Drinking water was supplied from a local source in pinhole type bottles (CLEA Japan, Inc., Tokyo, Japan) and autoclaved.

Housing Mouse R-PSF #CL-0132 cages were used for all strains as part of microisolation cage system (Safety Rack R, CLEA Japan, Inc., Tokyo, Japan).

Bedding Autoclaved paper bedding sheets, containing 75% soft and 25% hardwood pulps (CLEA Japan, Inc., Tokyo, Japan)

Photoperiod 12 hrs on (covering the hrs of 8:00 to 20:00) 12hrs off.

Ambient pressure +40 Pa (maximum positive pressure)

Temperature 23-25Â°C

Relative humidity 55-65%

Health status Mice were routinely monitored using the sentinel mouse program. See RIKEN BRC Quality Control website for the list of pathogenic agents monitored.

Supplier	RIKEN BioResource Center / Experimental Animal Division
Ages	Mice were housed in RIKEN BRC barrier facility until 8 weeks old when sample collection took place.
Diet	All strains maintained on a Gamma-irradiated commercial diet (CLEA Rodent Diet CE-2, Japan, Inc., Tokyo, Japan) CLEA
Water	Drinking water was supplied from a local source in pinhole type bottles (CLEA Japan, Inc., Tokyo, Japan) and autoclaved.
Housing	Mouse R-PSF #CL-0132 cages were used for all strains as part of microisolation cage system (Safety Rack R, CLEA Japan, Inc., Tokyo, Japan).
Bedding	Autoclaved paper bedding sheets, containing 75% soft and 25% hardwood pulps (CLEA Japan, Inc., Tokyo, Japan)
Photoperiod	12 hrs on (covering the hrs of 8:00 to 20:00) 12hrs off.
Ambient pressure	+40 Pa (maximum positive pressure)
Temperature	23-25Â°C
Relative humidity	55-65%
Health status	Mice were routinely monitored using the sentinel mouse program. See RIKEN BRC Quality Control website for the list of pathogenic agents monitored.