Acclimation and testing periods
Vendor Chromosome substitution strains were obtained by embryo transfer from Case Western Reserve University. CSS-1, -4, -5, -7, -10, -mt (mitochondria), and B57BL/6J were purchased from the Jackson Laboratory (JAX®, Bar Harbor, ME, USA). Ages Male mice, 7â9 weeks old, were tested between 10 am and 6 pm Feed Food (Prolab RMH3000) ad libitum, except during behavioral testing Water Autoclaved, supplied in plastic water bottles with sipper tubes ad libitum Housing Mice were housed 2â5 per cage of the same sex and strain in standard clear plastic cages (186 mm x 298 mm x 128 mm) with wire bar lid and filter top Bedding Steam autoclaved hardwood chips Photoperiod Mice were maintained on a 12-h light:dark cycle (12 hr on, 12 hr off; 7 am on - 7 pm off) Relative humidity ~50% Temperature 21-23°C Health status Mice were pathogen-free. Health status was routinely monitored through regular sentinel mouse testing to ensure no change in pathogen status. All experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH publication no. 86-23, 1996) and were approved by the Massachusetts Institute of Technology Committee on Animal Care.