Payseur2: Hybrid male sterility in F2 progeny from a WSB/EiJ x PWD/PhJ intercross (2018)

Schwahn DJ, Wang RJ, White MA, Payseur BA. Genetic Dissection of Hybrid Male Sterility Across Stages of Spermatogenesis. Genetics. 2018 Dec;210(4):1453-1465. doi: 10.1534/genetics.118.301658. Epub 2018 Oct 17.   PubMed 30333190     FullText

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Investigators Bret A Payseur       University of Wisconsin,  Madison, WI
Participants Schwahn DJ, Wang RJ, White MA
ContactBret A Payseur
Affiliated CenterQTL Archive
AcknowledgementsFunding provided by NIH GM120051 GM007133, NLM2M007359; NSF DEB1353737, DEB0918000; UW-Madison Science and Medicine Graduate Research Scholarship
Project type QTL Archive data set
MPD identifiersPayseur2     MPD:640
Data changelog No updates/corrections.       Initial release date: 01/2019.
Hybrid sterility is a common form of reproductive isolation between nascent species. Although hybrid sterility is routinely documented and genetically dissected in speciation studies, its developmental basis is rarely examined, especially in generations beyond the F1. To identify phenotypic and genetic determinants of hybrid male sterility from a developmental perspective, we characterized testis histology in 312 F2 hybrids generated by intercrossing inbred strains of Mus musculus domesticus and M.m. musculus, two subspecies of house mice. Hybrids display a range of histologic abnormalities that indicate defective spermatogenesis. Among these abnormalities, we quantified decreased testis size, reductions in spermatocyte and spermatid number, increased apoptosis of meiosis I spermatocytes, and more multinucleated syncytia. Collectively, our phenotypic data point to defects in meiosis I as a primary barrier to reproduction. We identified seven quantitative trait loci controlling five histologic traits. A region of chromosome 17 that contains Prdm9, a gene known to confer F1 hybrid male sterility, affects multinucleated syncytia, apoptosis of round spermatids, and round spermatid numbers.

QTL data set parameters:
Cross typeF2 Intercross
Population size 0 ♀     312 ♂

Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.

Project / data set:   Payseur2     a.k.a. Payseur_2018
Excel file:   Payseur2018_Data_Description.xlsx
CSV file:   Payseur2018_Data.csv