Payseur2: Hybrid male sterility in F2 progeny from a WSB/EiJ x PWD/PhJ intercross (2018)

Schwahn DJ, Wang RJ, White MA, Payseur BA. Genetic Dissection of Hybrid Male Sterility Across Stages of Spermatogenesis. Genetics. 2018 Dec;210(4):1453-1465. doi: 10.1534/genetics.118.301658. Epub 2018 Oct 17.   PubMed 30333190     FullText


       
Hybrid sterility is a common form of reproductive isolation between nascent species. Although hybrid sterility is routinely documented and genetically dissected in speciation studies, its developmental basis is rarely examined, especially in generations beyond the F1. To identify phenotypic and genetic determinants of hybrid male sterility from a developmental perspective, we characterized testis histology in 312 F2 hybrids generated by intercrossing inbred strains of Mus musculus domesticus and M.m. musculus, two subspecies of house mice. Hybrids display a range of histologic abnormalities that indicate defective spermatogenesis. Among these abnormalities, we quantified decreased testis size, reductions in spermatocyte and spermatid number, increased apoptosis of meiosis I spermatocytes, and more multinucleated syncytia. Collectively, our phenotypic data point to defects in meiosis I as a primary barrier to reproduction. We identified seven quantitative trait loci controlling five histologic traits. A region of chromosome 17 that contains Prdm9, a gene known to confer F1 hybrid male sterility, affects multinucleated syncytia, apoptosis of round spermatids, and round spermatid numbers.

Note on studied population: WSB/EiJ x PWD/PhJ intercross

Procedures conducted:
• study archive  Hybrid male sterility. Morphology, sperm count, histopathology.

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Investigators Bret A Payseur       University of Wisconsin,  Madison, WI
Participants Schwahn DJ, Wang RJ, White MA
ContactBret A Payseur     payseur@wisc.edu
Affiliated CenterQTL Archive
AcknowledgementsFunding provided by NIH GM120051 GM007133, NLM2M007359; NSF DEB1353737, DEB0918000; UW-Madison Science and Medicine Graduate Research Scholarship
Project type QTL Archive data set
MPD identifiersPayseur2     MPD:640
Data changelog No updates/corrections.       Initial release date: 01/2019.
QTL data set parameters:
Cross typeF2 Intercross
Population size 0 ♀     312 ♂
Age10-11wks


Measured phenotypes:       To access these data use the QTL Data Downloads button at bottom of page.
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Data set
Procedure / Protocol
Treatment
Phenotype Measure / Variable (click to view)
Panel
No. of Strains
Sex
Age
Sample Size (Avg)
Payseur2 organ weights (none) right testis weight adjusted for body weight   [mg/g]  RelRTestiswt   64001 QTL population m 10-11wks N=312
Payseur2 organ weights (none) right testis weight   [mg]  RTestisWt   64002 QTL population m 10-11wks N=312
Payseur2 sperm count (none) sperm count per mL (x106)   [n]  SpermDensity   64003 QTL population m 10-11wks N=312
Payseur2 organ dimensions (none) testis width   [mm]  TestisWidth   64004 QTL population m 10-11wks N=312
Payseur2 organ dimensions (none) testis height   [mm]  TestisHeight   64005 QTL population m 10-11wks N=312
Payseur2 histopathology (none) mulinucleated syncytia   [n]  MNSyncytiaPerTestis   64006 QTL population m 10-11wks N=312
Payseur2 histopathology (none) tubules with apoptosis   [n]  TubuleswApop   64007 QTL population m 10-11wks N=312
Payseur2 histopathology (none) spermatogonia with apoptosis   [n]  ApopGonia   64008 QTL population m 10-11wks N=312
Payseur2 histopathology (none) meiotic cells with apoptosis   [n]  ApopMeiotic   64009 QTL population m 10-11wks N=312
Payseur2 histopathology (none) round spermatids with apoptosis   [n]  ApopRS   64010 QTL population m 10-11wks N=312
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Project / data set:   Payseur2     a.k.a. Payseur_2018
Excel file:   Payseur2018_Data_Description.xlsx
CSV file:   Payseur2018_Data.csv