Parker1 animal documentation

Superovulation in 6 commonly used mouse strains   (2011)

Parker-Thornburg J
With: Luo C, Zuñiga J, Edison E, Palla S, Dong W

See also: Parker1 protocol


Animal documentation

Source C57BL/6NHsd, FVB/NHsd, B6D2F1/Hsd mice were obtained from Harlan Laboratories (Houston TX). B6(Cg)-Tyrc-2J/J mice were obtained from The Jackson Laboratory (Bar Harbor ME). BALB/cAnNCr AND Crl:CD-1(ICR) were obtained from Charles River Laboratories (Wilmington MA). Mice were used immediately if they were the appropriate weight or were bred in brother-sister matings (except Crl:CD-1(ICR)) for no more than 3 generations to obtain sufficient numbers of female mice of the desired weights. Crl:CD-1(ICR) were bred to unrelated males.
Ages
3-6 wks
Feed

Teklad Irradiated Diet 2919 (Harlan Laboratories, Madison WI, provided ad libitum

Water
Treated by reverse osmosis, provided ad libitum
Temperature
21 - 24°C
Humidity
Not specified
Housing
SPF conditions in static microisolation caging (Lab Products, Wilmington DE)
Health Status Pathogens excluded from the colony include mouse parvovirus, minute virus of mice, enzootic diarrhea of infant mice, Sendai virus, pneumonia virus of mice, reovirus 3, lymphocytic choriomeningitis virus, mouse adenovirus, ectromelia virus, K virus, and polyoma virus. Sentinels are screened quarterly.
Photoperiod 12:12h, lights on 0700h
Care and Use
All procedures approved by the University of Texas MD Anderson Cancer Center Institutional Animal Care and Use Committee