Metten1: Drug study: Assessment of ethanol-induced intoxication using a standard rating scale, in 9 inbred strains of mice (2004)

Metten P, Best KL, Cameron AJ, Saultz AB, Zuraw JM, Yu CH, Wahlsten D, Crabbe JC. Observer-rated ataxia: Rating scales for assessment of genetic differences in ethanol-induced intoxication in mice. J Appl Physiol. 2004 Jul;97(1):360-8. Epub 2004 Mar 19.   PubMed 15033960  


       
Metten1 downloads
• Download Metten1 project data set     one row per animal, one column per trait
• Download Metten1 strain means, SD, N, etc.     one row per strain/sex/measure
• Metten1 supplementary data
Participants Pamela Metten       Oregon Health & Science University Portland, OR
John C Crabbe       Oregon Health & Science University Portland, OR
Douglas Wahlsten       University of Windsor Windsor, Ontario CANADA
With: Best KL, Cameron AJ, Saultz AB, Zuraw JM, Yu C-H
ContactPamela Metten     mettenp@ohsu.edu
AcknowledgementsFunding provided by NIH AA10760, AA12714, AA13519; Department of Veterans Affairs.

Acknowledgments: Schlumbohm JP, Cotnam CJ, Forster A for technical assistance.
Project type Phenotype strain survey data set
MPD identifiersMetten1     MPD:165
Data changelog No updates/corrections.       Initial release date: 01/2007.
Formatted citation
Click above to copy-paste the entire citation for this MPD web page.
An investigation of mechanisms underlying ethanol-induced intoxication. Animals were scored using an observation-based rating scale of ataxia in combination with automated assessment of activity and motor function in an open field. Animals were measured at baseline and then again following ethanol administration at one of two randomly-assigned doses: (1) EtOH 2.75 g/kg body weight i.p.; (2) EtOH 3.0 g/kg body weight i.p.

Summary of procedures that were conducted
• behavior observation Observed gait, hindlimb splay 10 min and 30 min after ethanol i.p. (several doses).
• open field test Locomotor activity. Ethanol i.p. (several doses) vs. control (saline i.p.). 45s test.
Mice: inbred   9 strains   ♀♂   age 8-11wks   1 cohort

Data set MPD ID Procedure / Protocol Drug or Challenge Phenotype Measure Panel No. of Strains Sex Age Sample Size (Avg)
    Metten1 16501 open field test ethanol line crossings, 2.75 g/kg i.p.   [n]     pre-injection    10min after injection    30min after inbred 9 both 8-11wks N=4
    Metten1 16502 open field test ethanol line crossings, 3.0 g/kg i.p.   [n]     pre-injection    10min after injection    30min after inbred 9 both 8-11wks N=4
    Metten1 16509 behavior observation ethanol hindlimb splay, 2.75 g/kg i.p.   [score]     10min after injection    30min after inbred 9 both 8-11wks N=4
    Metten1 16511 behavior observation ethanol hindlimb splay, 3.0 g/kg i.p.   [score]     10min after injection    30min after inbred 9 both 8-11wks N=4
    Metten1 16513 behavior observation ethanol erratic gait, 2.75 g/kg i.p.   [score]     10min after injection    30min after inbred 9 both 8-11wks N=4
    Metten1 16515 behavior observation ethanol erratic gait, 3.0 g/kg i.p.   [score]     10min after injection    30min after inbred 9 both 8-11wks N=4
    All boxes