Mao1 project protocol
Motor performance and body weight in 16 Collaborative Cross strains of mice (2015)
Mao J, Snijders AM
With: Langley SA, Huang Y, Hang M, Bouchard KE, Celniker SE, Brown JB, Jansson JK, Karpen GH
Project
protocol
-
Contents
Workflow
and
sampling
Step |
Procedure |
Equipment |
Data
collected |
| 1 |
Weigh mice |
Scales |
Body weight |
2 |
Rotarod |
Accelerating rotarod |
Speed prior to falling off rotarod |
Equipment
and
supplies
- Scales for body weight
- Accelerating rotarod (spindle diameter 30 mm and lane dimension of 50 mm; 20 rpm/min)
Procedure: Accelerating rotarod
- All testing is conducted during the animal's light cycle.
- Mice are weighed on test day.
- Mice are placed on a rotarod spindle at a 45-degree angle.
- Mice are subjected to a slow-speed waiting mode (4 rpm for 5-10 s).
- Acceleration is started after the waiting period and set for 20 rpm/min for all mice.
- Animal falls are detected by a pressure sensitive lever, which automatically stops and records the speed at the time of the fall.
- Mice are tested consecutively an average of 3.3 times (range: 3-5).
- Repeat measurements of speed at the time of animal falling are averaged.
Data
collected
by
investigator
- body weight
- speed prior to falling off accelerating rotarod
|